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آنتی بادی مونوکلونال فلوسایتومتری CD39-PE ، کلون TU66

Name: Flow Cytometry Antibody CD39-PE, Clone TU66

  • Antibody CD39-PE is a monoclonal antibody (mAb) labelled with R-phycoerythrin (PE) designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of CD39 antigen-expressing cells.

SUMMARY AND EXPLANATION

FC is a powerful tool in analytical and quantitative characterization of cells which provides rapid and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. Flow cytometry is performed on cell suspension after incubating it with fluorescentlabelled antibodies directed against specific cellular proteins. Positive cells relative fluorescence intensity indicates the amount of antibody bonded to specific cell sites providing information about antigen expression.

CD39 is a cell surface protein member of the ecto-nucleoside triphosphate diphosphohydrolase (E-NTPDase) family. It is a 78 kDa cell surface enzyme also known as ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1). CD39 is expressed by regulatory T cells, mantle zone B cells, activated T cells, NK cells, macrophages, dendritic cells, neurons, endothelial cells and platelets. It is thought that CD39 allows Treg cells to enter inflamed areas where high levels of ATP are present. In the B-CLPD (B-cell chronic lymphoproliferative diseases) EuroFlow panel, CD39 is used to improve differential diagnosis between subgroups of lymphoplasmacytic lymphoma, marginal zone lymphoma, follicular lymphoma and diffuse large B-cell lymphoma cases.

REAGENT COMPOSITION

The purified monoclonal CD39 antibody conjugated with R-phycoerythrin (PE) is supplied in phosphate-buffered saline (PBS) containing 0.1% sodium azide.

  • Clone: TU66.
  • Isotype: IgG2b.
  • Amount per vial: 100 tests (5 µl mAb per determination).
  • Reagent is considered non-sterile.
  • یک آنتی بادی مونوکلونال (mAb) می باشد که به وسیله R-phycoerythrin (PE) نشاندار شده است.این آنتی بادی بعنوان یک معرف ایمنوفلوروسانس  مستقیم در شناسایی و شمارش سلول های که بیان کننده آنتی ژن CD39، به وسیله فلوسایتومتری استفاده می گردد.

             شماره کاتالوگ: CYT-39PE

            کلون:  TU66

ایزوتوپ: IgG2b

وضعیت: RUO

هدف: CD39

            فرم فیزیکی: مایع

  لیزر مورد نیاز برای برانگیختگی: Blue laser

 اندازه (تست):100 Test

 فلوروکروم: PE

شرایط نگهداری: در دمای 2-8  درجه سانتی گراد و مکان خشک نگهداری شود و همچنین معرف نباید فریز گردد و یا در معرض نور مستقیم خورشید قرار بگیرد.

 

 

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