آنتی بادی مونوکلونال فلوسایتومتری CD38-PE ، کلون T16

Name: Flow Cytometry Antibody CD38-PE, Clone T16

• Antibody CD38-PE is a monoclonal antibody (mAb) labelled with R-phycoerythrin (PE) designed for Flow Cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of human CD38 antigen-expressing cells. This reagent must be used by FC qualified personal.

SUMMARY AND EXPLANATION

CD38 antigen is a type II transmembrane glycoprotein expressed at variable levels on the majority of hematopoietic cells (during early differentiation and activation) and in some non-hematopoietic tissues. It is expressed at high levels on plasma cells (CD38+++) and, due to its strong reactivity, CD38 is considered a “specific plasma cell marker”; but it is also present on thymocytes, activated T cells, NKs monocytes, and basophils.. CD38 has been reported to play a role as regulator (positive and negative) of cell activation and proliferation, and to be involved in adhesion between lymphocytes and endothelial cells. CD38 antibody is used in FC for diagnosis and monitoring of plasma cell disorders (e.g. multiple myeloma, MGUS, plasma cell leukemia, etc.). The simultaneous assessment of the expression of CD38 and CD138 represents the best combination of markers for the identification of plasma cells. Plasma cells (CD38+++) are expressed as a percentage of leucocytes present in a sample. Since every flow cytometer has different operating characteristics each laboratory must determine its optimal operating procedure and the combination of markers used to identify the target cell population.

REAGENT COMPOSITION

The purified monoclonal CD38 antibody conjugated with R-phycoerythrin (PE) is supplied in phosphate-buffered saline (PBS) containing 0.09% (m/v) sodium azide.

• Clone: T16.
• Isotype: IgG1.
• Size: 200 tests (1 ml per vial).
• Usage: 5 µl mAb per determination.
• Reagent is considered non-sterile