آنتی بادی مونوکلونال فلوسایتومتری CD25-APC ، کلون M-A251

Name: Flow Cytometry Antibody CD25-APC, Clone M-A251

• Antibody CD25-APC is a monoclonal antibody (mAb) labelled with allophycocyanine (APC) designed for flow cytometry use as a direct immunofluorescence reagent in the identification and enumeration of CD25 antigen-expressing cells. This reagent must be used by flow cytometry qualified personal.

SUMMARY AND EXPLANATION

CD25 antigen is part of interleukin-2 receptors. These receptors consist of three polypeptides (α, β, γ), which in different combinations bind IL-2. The 55 kDa α chain is called CD25. The α chain alone, the βγ heterodimer, and the αβγ heterotrimer form the low-, intermediate- and high affinity IL-2R, respectively. The intermediate- and high affinity IL-2R play a role in intracellular signal transduction. CD25 is strongly expressed on the surface of T cells activated by antigens, mitogens (phytohemagglutinin A (PHA), concanavalin A (Con-A), pokeweed mitogen (PWM)), Staphylococcus aureus, viruses (HTLV-I, HTLV-II, Epstein-Barr virus), and is also expressed on B cells stimulated with anti-IgM antibody, and on monocytes/macrophages stimulated with lipopolysaccharide. In normal peripheral blood a variable staining of lymphoid cells (about 3-25%) is observed.

REAGENT COMPOSITION

The purified monoclonal CD25 antibody conjugated with allophycocyanine (APC) supplied in phosphate buffered saline with 0,09% sodium azide and 0.2% (w/v) bovine serum albumin (BSA).

• Clone: M-A251.
• Isotype: IgG1.
• Amount per vial: 100 tests 5 µl mAb per determination.
• Reagent is considered non-sterile.