Name: Mouse Anti-Human Keratin AE1-AE3 Cocktail Antibody clone AE1-AE3
Description and applications: The nomenclature coined in 1982 by Moll and Franke assigned ranges from 1 to 8 for keratin type II (neutral or basic) and between 9 and 21 for type I (acidic). However and because of the high homology among the different molecules is common for a single monoclonal antibody to react with different types of keratins. This antibody, comprising a cocktail of AE1 and AE3 monoclonal antibodies, simultaneously recognizes keratins 10, 14, 15, 16 and 19 and 1, 2, 3, 4, 5, 6, 7 and 8, so that it is considered as one of the broadest spectrum reagents for the identification of epithelial origin in a given neoplasm. However, it should be borne in mind that besides hepatocarcinomas other tumors that often can be negative to the antibody AE1-AE3 are kidney, adrenal, prostate and neuroendocrine carcinomas. For these reasons the AE1-AE3 keratin antibody should be used in conjunction with other broad spectrum keratins.
Composition: anti-Keratin AE1-AE3 mouse antibody obtained obtained from mouse ascites by protein A chromatography in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal. The quantity of the active antibody in the sample is of approximately 4 μ/mL..
Intended use : Immunohistochemistry (IHC) on paraffin embedded tissues. Not tested on frozen tissues or Western-Blotting
Immunogen: Human epidermal cells