.(Name: Mouse anti-human Caldesmon Monoclonal Antibody clone (h-CALD
Description and applications: The antibody recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120–150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70–80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150kDa variant (hcaldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.This antibody may be useful for the study of muscle contraction and as a useful marker of neoplasms of smooth muscle origin. It is not expressed in myofibroblastic cells or tumors derived from them. Nodular fasciitis, fibromatosis and myofibroblastic sarcomas are negative for h-caldesmón.
COMPOSITION: Anti-human Caldesmon mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
Immunogen: Crude human uterus extract.