کیت 2Steps Detection System Goat Anti-Mouse / Rabbit HRP + DAB برند Patho Sage

2 Steps Detection System Peroxidase Goat Anti-Mouse/ Rabbit HRP + DAB is intended for use in immunohistochemistry for the detection of mouse or rabbit antibodies.

Summary and explanation:
The 2 Steps Detection System Peroxidase Goat Anti-Mouse/ Rabbit HRP + DAB, is a Ready-to-Use system that has been manufactured to give an optimal staining, when using the protocol advised in this IFU.
Prior to staining some routine formalin fixed, paraffin-embedding(FFPE)tissue sections should be subjected to pre-treatment (HIER or digestive enzyme). The 2 Steps Detection System detects Mouse or Rabbit bound to an antigen in tissue sections. The antibodies are not provided but it is recommended to use the PathoSage-antibodies.This polymer-complex is then visualized with a suitable substrate/chromogen. The clinical interpretation of any staining or its absence should be determined by a qualified pathologist and complemented by morphologic studies; controls should be evaluated within the context of the patient’s clinical history and/or other diagnostic tests.

Kit component:

2 Steps Detection System Goat Anti-Mouse/ Rabbit HRP + DAB

1. Post-blocking (ready-to-use)
2. Polymer Goat Anti-Mouse/Rabbit HRP (Ready-to-Use)
3. DAB Solution A: Buffered H2O2 (Ready-to-Use)
4. DAB Solution B: Concentrated DAB solution