دسته بندی: آنتی بادیهای ایمونوهیستوشیمی

 Name: Pan Melanoma Cocktail Antibody clone HMB45+M2-7C10+M2-9E3

Description and applications: The antibody represents a cocktail of anti-gp100 (HMB45) and MART-1 antibodies. By
immunohistochemistry, it recognizes melanocytes and melanomas. This cocktail reacts with junctional and blue nevus cells and with fetal and neonatal melanocytes. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. The antibody labels melanotic as well as amelanotic variants of melanomas. It is extremely sensitive for labeling formalin-fixed, paraffinembedded melanomas and other tumors showing melanocytic differentiation.

Composition: Anti-human Pan Melanoma Cocktail mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide

Name: Pancreatic lipase Antibody clone EPR6275

Description and applications: Pancreatic lipase is a 56kDa molecular mass enzyme actively secreted in the duodenum by the pancreas in the presence of bile salts and colipase. It belongs to the family of triglyceride lipases, of which there are at least three tissue-specific isoenzymes (pancreatic, hepatic and gastric and lingual) that break down dietary fats by converting triglycerides into monoglycerides and free fatty acids. Pancreatic lipase together with bile salts favors the absorption into the lymphatic system of the monomers resulting from digestion. Human pancreatic lipase is encoded by the PNLIP gene of 13 exons and more than 20 kb, located on the chromosomal region 10q25.3. In normal tissues this antibody identifies pancreatic acinar cells and in neoplasms it recognizes acinary cell carcinoma of the pancreas, which expresses this marker in more than 50% of cases. For this reason and integrated in a panel of suitable antibodies, pancreatic lipase allows the differentiation of acinar adenocarcinoma of pancreas from other malignancies with similar morphology (especially neuroendocrine carcinoma with acinary architecture) and establish the pancreatic origin of distant metastases, that on numerous occasions may be the first manifestation of the tumor.

Composition: Anti-human Pancreatic lipase mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide

Name:Cytokeratin Antibody clone EP24/EP67/B22.1&B23.1

Description and applications: This antibody recognises cytokeratins 5, 6, 8, and 18. Proteins generically called intermediate filaments, because they measure between 7 and 22 nm in diameter (i.e., with a size between actin, 5-7 nm, and tubulin, 22-25 nm), are part, along with actin and tubulin, of the vertebrate cytoskeleton. This superfamily is composed of six subfamilies of molecules with different tissue expression patterns. Cytokeratins constitute homology groups I and II, and in humans they are encoded by more than 49 different genes located in chromosomes 17 (I) and 12 (II). The nomenclature chosen in 1982 by Moll and Franke assigns numbers from 1 to 8 to type II cytokeratins (neutral or basic) and from 9 to 21 to type I cytokeratins (acidic). An analogous nomenclature for hair keratins has now been defined, with the addition of letters Ha and Hb to distinguish type I from type II. Structurally, cytokeratins share with the rest of intermediate filaments a central axis of 310 amino acid residues consisting of four highly preserved α-helical domains (1A, 1B, 2A, and 2B) that define the type of intermediate filament that will be formed after assembly; these domains are separated by three non-helical linking regions (L1, L12, and L2) and two end domains which are highly different in size and sequence (head, 1, and tail, 2), each with constant (E1/E2), variable (V1/V2), and homology (H1/H2) regions, the latter characteristic of type II keratins and absent in type I keratins. The major immunogenic properties and the main differences between each keratin class are found in the variable domains. Keratins are usually assembled into I/II heterodimers and are specifically co-expressed in pairs in each tissue. Due to the high homology among the different molecules, it is common for a monoclonal antibody to react with different types of cytokeratins; for example, anti-cytokeratin AE1 labels cytokeratins 10, 14, 15, 16, and 19. The anti-cytokeratin monoclonal antibody cocktail is a broad-spectrum antibodt for general use. This antibody mixture reacts with carcinomas in general, but as with other anti-cytokeratin antibodies, it is possible that poorly differentiated carcinomas occasionally exhibit poor immunostaining. Its most common application is the differential diagnosis of malignant tumours, in which case it can be used within a panel of antibodies, such as anti-ALC, which recognises lymphoma, or anti-S100 or HMB45, which labels malignant melanoma. The staining of other non-carcinoma tumours is uncommon, although as with other anti-cytokeratin 8 antibodies, smooth muscle and leiomyoma labelling may occasionally be observed.

Composition: Anti-human Cytokeratin 5/6/8/18 rabbit/mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide

Name:Antibody Calponin clone EP63

Description and applications: Calponin is a smooth muscle specific, actin-, tropomyosin- and calmodulin-binding protein thought to be involved in regulation of actomyosin as well as the regulation or modulation of contraction. It is expressed on smooth muscle cells and myoepithelial cells. Calponin antibody has been used to identify invasion of breast lesion. Additionally, Calponin is expressed on malignant fibrous histiocytoma of bone and adenoid cystic carcinoma of salivary gland. The consistently positive staining pattern in adenoid cystic carcinomas may be useful in discriminating

Composition: Anti-human Calponin rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide

Name: Epithelial Specific Antigen-Clone Ber-EP4

Description and aplications: This antibody reacts with two glycoproteins of 34 and 49 kD molecular weight present on the surface and in the cytoplasm of all epithelial cells except the surface layer of the squamous epithelium, hepatocytes and parietal cells of the stomach. This antibody is specific to HEA125 equivalent. The antibody shows a broad pattern of reactivity with human epithelial tissues like simple epithelium, basal layer of the stratified squamous non-keratinized epithelia and epidermis. In contrast to other known anti-epithelial antibodies, this antibody does not mark the mesothelial cells and only a small number of mesotheliomas stained positively. This antibody does not react with nervous, glial, muscle, mesenchymal or lymphoid tissue. Due to the labile nature of the epitope in tissues fixed in buffered formalin and embedded in paraffin, negative staining results should be considered with caution.

Composition: anti-Ber-EP4 mouse monoclonal antibody obtained from supernatant culture and prediluted in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal.

Immunogen: MCF-7 human breast carcinoma cell line.

Name: Epstein-Barr Virus / LMP1   (Clone CS1-4)

Description and applications:The antibody strongly reacts with EBV-positive lymphoblastoid cell lines and EBV infected B cell immunoblasts in infectious mononucleosis. It also reacts with 25 to 50 per cent of EBV associated undifferentiated nasopharyngeal carcinomas and with Reed Sternberg cells in approximately 90% of EBVassociated Hodgkin’s disease cases. The cocktail recognizes distinct epitopes on the hydrophilic carboxyl region of LMP which is exposed to the cytosol.

Composition:Anti-human Epstein-Barr Virus / LMP1 mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: EBV-encoded recombinant latent membrane protein.

Name: Fascin  (Clone FCN01)

Description and applications:Anti-Fascin is a very sensitive marker for ReedSternberg cells and variants in nodular sclerosis, mixed cellularity, and lymphocyte depletion Hodgkin’s disease. It is uniformly negative in lymphoid cells, plasma cells and myeloid cells. Anti-Fascin is positive in dendritic cells. This marker may be helpful in distinguishing between Hodgkin’s disease and nonHodgkin’s lymphoma in difficult cases. Also, the lack of expression of Fascin in the neoplastic follicles in follicular lymphoma can be helpful in distinguishing these lymphomas from reactive follicular hyperplasia in which the number of follicular dendritic cells is normal or increased. Anti-Fascin has been suggested as a prognostic marker in neuroendocrine neoplasms of the lung, as well as ovarian cancer.

Composition: Anti-human Fascin mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Name: Glutamine Synthetase  (Clone GS-6)

Description and applications:Glutamine synthetase (GS) catalyzes the synthesis of glutamine from glutamate and ammonia in the mammalian liver. In normal liver, GS expression is seen in pericentral hepatocytes, but not in mid-zonal or periportal hepatocytes. Glutamine, the end product of GS activity, is the major energy source of tumor cells. Based on findings from experimental hepatocarcinogenesis, GS positive tumor cells are believed to be derived from GS positive hepatocytes. Thus, anti-GS has been suggested as a marker for hepatocellular carcinoma (HCC). GS immunoreactivity has been seen in a majority of HCC (37 of 53 cases, 70%), including 7 of 10 cases of early HCC (70%) and 12 of 22 (59%) for low grade HCC. In nonmalignant nodules, GS overexpression was only seen in 3 high grade dysplastic nodules (HGDN,13.6%). In these cases, GS overexpression was restricted to 11.5%-50% of hepatocytes, whereas in HCC the majority of cases (28 of 53, 53%), including early HCC (60%), showed diffuse immunostaining (>50% tumor cells). Overall, the sensitivity, specificity, and positive and negative predictive values of anti-GS for HCC detection were 69.8%, 94.2%, 92.5%, and 75.4%, respectively. A panel composed of antibodies against HSP70, GPC3, and GS has been proposed to be very useful in distinguishing between dysplastic and early malignant hepatocellular nodules arising in cirrhosis. The “all positive” phenotype is restricted to approximately half of early HCC to well-differentiated HCC but has never been reported in dysplastic lesions, whereas the reverse phenotype, “all negative”, has been shown to be a feature of the majority of HGDN and of all low grade dysplastic nodules.

Composition: Anti-human Glutamine Synthetase mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

name: PAX-2 Antibody clone EP235

Description and aplications: PAX2 is a member of the paired box family of transcription factors, which is required for development and proliferation of the kidney, brain, and müllerian organs. PAX2 genes contain a highly conserved DNA sequence within the paired box region, which encodes a DNA-binding domain, enabling PAX proteins to bind the promoters of specific genes to transcriptionally regulate their expression. Defects in PAX2 gene are related with the renal coloboma syndrome (RCS) (also known as papillorenal syndrome) which is a condition that primarily affects kidney (renal) and eye development. PAX2 is specifically expressed in the developing central nervous system, eye, ear, and urogenital tract, and is essential for the development of these organs. In normal adult tissues PAX2 was mainly detected in the urogenital system, including kidney, uretericepithelium, fallopian tube epithelium, ovary and uterus. In tumors, PAX2 has been detected in renal cell carcinomas, Wilms’ tumors, nephrogenic adenomas and papillary serous carcinoma of the ovary. For these reasons, PAX2 has been used as a marker for the identification of renal cell carcinoma and ovarian carcinoma by immunohistochemistry. It has been also suggested as a useful tool in diagnosis and classification of hyperplastic endometrial epithelial proliferations

Composition: anti-human PAX2 rabbit monoclonal antibody purified from ascites fluid by chromatography. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide