دسته بندی: مونوکلونال

Name: Antibody AFP(alpha-fetoprotein) clone EP209

Description and applications: Alpha-fetoprotein (AFP) is the most abundant plasma protein found in the human fetus. It is thought to be the fetal form of serum albumin. AFP binds to copper, nickel, fatty acids and bilirubin and is found in monomeric, dimeric and trimeric forms. Alpha-Fetoprotein (AFP) is synthesized by the cells of the embryonic yolk sac, fetal liver and fetal intestinal tract. AFP levels decrease soon after birth. In abnormal tissues, expression of AFP has been demonstrated in hepatocellular carcinoma, hepatoid adenocarcinoma, germ cell tumors, particularly yolk sac tumor. The anti-AFP antibody may be useful for the identification of neoplastic liver diseases or yolk sac tumors.

Composition: anti-human AFP rabbit monoclonal antibody purified by protein affinity and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide

Immunogen: A synthetic peptide corresponding to residues of human AFP protein

Name: Amyloid-A Monoclonal Antibody

Description and applications: Este anticuerpo reacciona con depósitos de amiloide de tipo AA, tanto la forma nativa como desnaturalizada de la proteína fibrilar. Este anticuerpo reacciona con depósitos de amiloide en todos los órganos y tejidos, así como con el precursor sérico de la proteína AA.

Composition: anti-Amyloid-A mouse monoclonal antibody obtained from supernatant culture and prediluted in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal.

Name: CD23 Monoclonal Antibody clone SP23

Description and applications: The role of IgE immunoglobulins in the initiation and development of acute allergic processes is well known. These processes are generally initiated through its interaction with high affinity receptors for Fc portion (FceRI) located mainly on the surface of mast cells and basophil granulocytes where it regulates exocytosis phenomena and subsequent degranulation of mediators of hypersensitivity reactions type I. Furthermore, the immunoglobulin IgE also binds specifically to an integral membrane glycoprotein of 45- 55 kDa type 2 (321 aa) that does not belong to the immunoglobulin superfamily but to the selectins and are included under the name of low affinity receptor of IgE (FceRIIa+b / CD23). CD23 is expressed in a wide range of cell types, including B lymphocytes, eosinophils, granulocytes,  platelets, T and NK cells, dendritic reticulum cells and Langerhans cells. CD23 expression has been detected in neoplastic lymphoid cells of chronic lymphocytic leukemia, in a variable percentage of follicular lymphomas and in a small proportion of large B-cell lymphomas. Furthermore and although the CD23 molecule is produced by B cells, is adsorbed in a large amount on follicular dendritic reticulum cells thus is also used for the identification of its neoplasms (dendritic reticulum cell sarcoma).

Composition: anti-CD23 mouse monoclonal antibody obtained from supernatant culture and prediluted in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal.

Immunogen: Recombinant external domain of CD23 protein.

(Name: Mouse anti-CMV (Cytomegalovirus) cocktail of Monoclonal Antibodies (Clone DDG9/CCH2

Description and aplications: Cytomegalovirus is a member of the herpes virus group, which includes herpes simplex virus types 1 and 2, varicella zoster virus (which causes chicken pox), and Epstein Barr virus (which causes infectious mononucleosis). These viruses share a characteristic ability to remain dormant within the body over a long period.This antibody cocktail reacts with an immediate early antigen and an early CMV antigen. DDG9 is isotype IgG2a, kappa whilst CCH2 is isotype IgG1, kappa. The antibodies stain infected cells giving a nuclear staining pattern with an immediate early and an early antigen.

Composition: anti-CMV is a cocktail of two mouse monoclonal antibodies from tissue culture supernatant diluted in tris buffered saline, pH 7.3-7.7, with protein base, and preserved with sodium azide.

.Immunogen: Cytomegalovirus-infected cell lysate

(Name: Mouse anti-human CA125 (Mucin-16) Monoclonal Antibody (Clone OC125

Description and applications: CA 125 (Cancer Antigen 125) is a mucin-like glycoprotein with a molecular weight greater than 200 kD. OC 125 recognizes an epitope that is considered to be a peptide in nature. Anti-CA-125 antibody reacts with epitheloid malignancies of the ovary, papillary serous carcinoma of the cervix, adenocarcinoma of the endometrium, clear cell adenocarcinoma of the bladder, and epitheloid mesothelioma. The antigen is formalin resistant, permitting the detection of ovarian cancer by immunohistochemistry, although serum assays for this protein are widely used to monitor ovarian cancer. CA 125 is located on the surface of ovarian tumor cells with essentially no expression in normal adult ovarian tissue. CA125 is also found in sera of patients with pancreatic, liver, colon, and other adenocarcinomas.1 These references are for presentation in vials of Low Density Polyethylene (LDPE) dropper. In case the products are used in automated stainers, a special reference is assigned as follows: – / L: Cylindrical screw-cap vials (QD-3 / L, QD-7 / L, QD-12 / L). – / N: Polygonal screw-cap vials (QD-3 / N, QD-7 / N, QD-12 / N). For different presentations (references / volumes) please contact the supplier. CA 125 is found in the epithelial cells of normal colon, gall bladder, mammary gland, and stomach. Normal breast, liver, skin, and spleen are usually negative. When used with other antibodies, CA 125 antibodies are useful for the separation of colonic carcinoma from ovarian endometroid carcinoma in the pelvis and distinguishing renal clear cell carcinoma from clear cell carcinoma of the ovary and other Mullerian tumors.

Composition: anti-human CA125 mouse monoclonal antibody purified from ascites. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: Ovarian cancer cell line OVCA433 derived from patient with serous papillary cystadenocarcinoma.

.(Name: Mouse anti-human Calcitonin Monoclonal Antibody (Clone SP17

Description and applications: Calcitonin is a 32 amino acid peptide which can be demonstrated in C cells of the normal and hyperplastic thyroid. Staining for calcitonin may be used for the identification of a spectrum of C cell proliferative abnormalities ranging from C cell hyperplasia to invasive tumors. Staining for calcitonin in medullary carcinoma of the thyroid produces a fine granular pattern in the cytoplasm. Amyloid deposits within the tumor may also exhibit varying degrees of calcitonin activity.

Composition: anti-human Calcitonin rabbit monoclonal antibody purified from ascites fluid by Protein A chromatography. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: Synthetic human calcitonin 1-32 amino acid peptide.

(Name: Rabbit anti-human Carbonic Anhydrase 9 Monoclonal Antibody (Clone EP161.

Description and applications: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and subcellular localization. Carbonic Anhydrase 9, a member of the carbonic anhydrase family, is thought to play a role in the regulation of cell proliferation in response to hypoxic conditions and may be involved in oncogenesis and tumor progression.Carbonic Anhydrase 9 (CA9) has a distinctive expression pattern in normal and cancer tissues. The most abundant expression of CA9 was found in normal mucosa of the stomach and gallbladder. Other normal tissues have lower or no expression. Relatively high levels of CA9 are expressed in carcinomas of the cervix, kidney, lung, breast and many other tumors. Most studies have shown that decreased CA9 levels are independently associated with poor survival. Low levels of CA9 maybe benefit more from adjuvant treatment than patients with high levels.

COMPOSITION: Anti-human Carbonic Anhydrase 9 rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

IMMUNOGEN: A synthetic peptide corresponding to residues in the extracellular domain in human Carbonic Anhydrase 9 was used as an immunogen.

.(Name: Mouse anti-human Cathepsin K Monoclonal Antibody (Clone 3F9

Description and Applications: Cathepsin K is a lysosomal papain–like cystine proteinase and can be expressed in normal osteoblasts, fibroblasts, and skin. Recently, cathepsin K has been reported as useful in distinguishing translocation renal cell carcinoma (RCC) from other subtypes of RCC. Mortignoni et al. tested 17 cases of translocation RCC and found that cathepsin K stained 6 of 8 cases of translocation RCC with t(X;1), all seven cases of translocation RCC with t(6;11), but was negative for one case with t(X;3) and one with t(X;17).1 Additionally, the study compared IHC detection of TFE3 antibody in the 17 cases and demonstrated that all translocation RCC with t(6;11) were completely negative for TFE3, but positive for cathepsin K; all other translocation RCC with different chromosomal tranlocations other than t(X;1) expressed TFE3. Thus, it is recommended that antibodies against both TFE3 and cathepsin K should be used for evaluating translocation renal cell carcinomas. None of the 305 other renal cell neoplasms representing the most common renal cell neoplasm subtypes were positive for cathepsin K. Zheng et al. reported only 2.7% of 1,140 carcinomas from various organs exhibited cathepsin K labeling, indicating that among carcinomas cathepsin K labeling is highly specific for translocation RCC and can be used to help differentiate translocation RCC from other carcinomas.

COMPOSITION: Anti-human Cathepsin K mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

.(Name:  Mouse Anti-Glycophorin A Monoclonal Antibody (Clone GA-R2

 Description and aplications: This antibody reacts with a resistant paraffin-embedded and formol fixed epitope located in the extracellular domain of the protein, probably between amino acids 27 and 40. Therefore, this antibody recognizes normal erythroid cells in all stage of differentiation from erythroblasts to mature red blood cells. Once glycophorin A is at its maximum expression, the quantity in each erythroid cell remains constant and shows no change during maturation despite the decreasing size of the cell. The neoplastic erythroblast in most erythroleukemias are identified by Glycophorin A.

Composition: anti-Glycophorin A mouse monoclonal antibody obtained from supernatant culture and prediluted in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal.

Immunogen: membrane preparation from splenic hairy cell leukemia cells.

Name: Mouse Anti-Human Keratin AE1-AE3 Cocktail Antibody clone AE1-AE3

Description and applications: The nomenclature coined in 1982 by Moll and Franke assigned ranges from 1 to 8 for keratin type II (neutral or basic) and between 9 and 21 for type I (acidic). However and because of the high homology among the different molecules is common for a single monoclonal antibody to react with different types of keratins. This antibody, comprising a cocktail of AE1 and AE3 monoclonal antibodies, simultaneously recognizes keratins 10, 14, 15, 16 and 19 and 1, 2, 3, 4, 5, 6, 7 and 8, so that it is considered as one of the broadest spectrum reagents for the identification of epithelial origin in a given neoplasm. However, it should be borne in mind that besides hepatocarcinomas other tumors that often can be negative to the antibody AE1-AE3 are kidney, adrenal, prostate and neuroendocrine carcinomas. For these reasons the AE1-AE3 keratin antibody should be used in conjunction with other broad spectrum keratins.

Composition: anti-Keratin AE1-AE3 mouse antibody obtained obtained from mouse ascites by protein A chromatography in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal. The quantity of the active antibody in the sample is of approximately 4 μ/mL..

Intended use : Immunohistochemistry (IHC) on paraffin embedded tissues. Not tested on frozen tissues or Western-Blotting

Immunogen: Human epidermal cells

.(Name: Mouse anti-human Cytokeratin5/6 Monoclonal Antibody (Clone EP24/EP67

Description and applications: Keratins are cytoplasmic intermediate filament proteins expressed by epithelial cells. CK5 is a type II cytokeratin. Loss-of-function mutations in the keratin 5 gene (KRT5) affected family members and in six unrelated patients with Dowling-Degos disease (DDD), an autosomal  dominant genodermatosis. This suggests a crucial role for keratins in the organization of cell adhesion, melanosome uptake, organelle transport, and nuclear anchorage. CK5 labels myoepithelial cells of breast and prostate basal cells. CK5 and calretinin have been useful in different studies as immunohistochemical markers suggestive of mesothelioma, and their expression is analyzed for the histological differentiation with adenocarcinomas, especially when confronting metastatic tumors of unknown origin. The human type II Cytokeratin 6 (CK6; 56 kDa) is well known for its strong induction in stratified epithelia that feature an enhanced cell proliferation rate or abnormal differentiation during wound healing, in several diseases (e.g. psoriasis, actinic keratosis) and cancer. CK6 is expressed on stratified epithelia including oral mucosa, esophagus, basal layer of epidermis, the outer root sheath of hair follicles, and in glandular epithelia. CK6 is a marker of hyperproliferative and activated keratinocytes found in psoriasis.Anti-CK6 paired with the CK5 antibody is useful for differentiating mesothelioma (positive) from lung carcinoma (negative) or metastatic carcinoma (negative) in the pleura. An antibody against CK5/6 has also been used to distinguish usual ductal hyperplasia of the breast (strong staining) from solid papillary DCIS (negative).

Immunogen: A synthetic peptide.

Name: Rabbit anti-human Gross Cystic Disease Fluid Protein-15 (BRST-2/GCDFP-15) Monoclonal Antibody (Clone EP95).

Description and Applications: Gross cystic disease fluid protein (GCDFP-15), also called prolactininducible protein (PIP), is a single polypeptide chain with a versatile function in human reproductive and immunological systems. GCDFP-15 binds to CD4, exerts a potent inhibition on T lymphocyte apoptosis mediated by CD4/T-cell receptor (TCR) activation, and carries a fibronectinspecific aspartyl protease activity. It is up regulated by prolactin and androgens, while it is down regulated by estrogen. In normal adult tissues, GCDFP-15 expression was found in all apocrine, lacrimal, ceruminous, and Moll’s glands and in numerous serous cells of the submandibular, sublingual, and minor salivary glands. The serous cells of nasal and bronchial glands were also positive. It is used as a marker of apocrine differentiation. GCDFP-15 has been found in the cyst fluid of cystic breast disease and primary and metastatic breast cancer, and considered a highly specific marker for identification of breast cancer. GCDFP-15 expression has also been found in other cancer types including salivary glands, sweat glands, prostate, and lung.

COMPOSITION: Anti-human BRST-2/GCDFP-15 rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

IMMUNOGEN: A synthetic peptide corresponding to residues in human Gross Cystic Disease Fluid Protein15 (GCDFP-15) protein.