دسته بندی: مونوکلونال
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی Smooth Muscle Actin کلون 1A4 برند Patho Sage
Specification:
Actin is a major component of the cytoskeleton and is present in every cell type. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti Smooth Muscle Actin antibody does not stain cardiac or skeletal muscle; however, it will stain myofibroblasts and myoepithelial cells. This antibody could be used together with Muscle Specific Actin to distinguish leiomyosarcoma from rhabdomyosarcoma. In most cases of rhabdomyosarcoma, this antibody gives negative results whereas Muscle Specific Actin is positive in the rhabdomyoblasts. Leiomyosarcomas are positive with both Muscle Specific Actin and Smooth Muscle Actin antibodies.
Presentation:
Bioreactor Concentrate with 0.05% Azide.
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی BCL2 کلون 124 برند Patho Sage
Specification:
Anti-BCL2 has shown consistent negative reaction on reactive germinal centers and positive staining of neoplastic follicles in follicular lymphoma. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. This antibody may also be used in distinguishing between those follicular lymphomas that express BCL2 protein and the small number in which the neoplastic cells are BCL2 negative. Anti-BCL2 has been used as a predictive biomarker for recurrence in the case of radical prostatectomy for prostate cancer and has shown promise as a prognostic marker in the case of cancer of the breast and non-small cell carcinoma of the lung.
Immunogen:
Synthetic peptide corresponding to Bcl-2 aa 51-54
Presentation:
Bioreactor Concentrate with 0.05% Azide
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی CDX2 کلون EPR2764Y برند Patho Sage
Specification:
CDX-2 is a caudal-related homeobox transcription factor whose expression in the adult is normally restricted to the intestinal epithelium. It is implicated in the development and maintenance of the intestinal mucosa. This protein is expressed immunohistochemically in the nuclei of normal intestinal epithelium. Loss of CDX-2 protein expression has been correlated with loss of differentiation in colorectal cancers. Anti CDX-2 antibody has been useful in distinguishing gastrointestinal origin of metastatic adenocarcinomas and carcinoids. A high percentage of mucinous carcinomas of the ovary also stain positively with this antibody as well as carcinomas from the upper gastrointestinal tract.
Immunogen:
Synthetic peptide corresponding to residues near the N-terminus of human CDX-2
Presentation:
1.0 ml tissue culture supernatant with 0.1% sodium azide.
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی GATA3 کلون L50-823 برند Patho Sage
Specification:
GATA-3 (GATA binding protein 3) is a member of the GATA family of transcription factors. This 50 kD a nuclear protein regulates the development and subsequent maintenance of a variety of human tissues, including hematopoietic cells, skin, kidney, mammary gland and the central nervous system. Among several other roles, GATA-3 involved in luminal cell differentiation in the mammary gland and appears to control a set of genes involved in the differentiation and proliferation of breast cancer. The expression of GATA-3 is associated with the expression of estrogen receptor-alpha (ER) in breast cancer. GATA-3 has been shown to be a novel marker for bladder cancer. The study demonstrated that GATA-3 stained 67% of urothelial Carcinomas, but none of prostate or renal carcinomas.
Immunogen:
Conserved peptide between the GATA trans-activation and DNA-binding domain
Presentation:
Purified antibody in 0.2% BSA and 15mM sodium azide.
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی CD20 کلون L26 برند Patho Sage
Specification:
This antibody reacts with a formalin-resistant intracytoplasmic epitope found in the majority of B cells and now considered to be the CD20 antigen, a pan-B cell marker. The antibody primarily recognizes a 33 kD polypeptide B cell component and also a minor 30 kD cellular antigen. The staining pattern is consistent with pan-B reactivity, producing staining for B cells in lymphoid and peripheral blood tissue. This antibody intensely stains germinal centers and B immunoblots in lymphoid tissue. The L26 antibody is widely regarded as the most selective B cell marker currently available. L26 has shown no cross reactivity with nonlymphoid tissues and there are several studies demonstrating its sensitivity and specificity in formalin fixed, paraffin-embedded tissues. Studies have demonstrated that, in cases of lymphocyte predominant Hodgkin’s lymphomas, L&H variants of Reed-Sternberg cells show a strong L26 reactive pattern that is distinctive from other Reed Sternberg variants. L26 may prove to be a useful marker for this particular subset of Hodgkin’s lymphomas.
Immunogen:
Human tonsil B cells
Presentation:
Bioreactor concentrate with 0.05% Azide
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی Cytokeratin Cocktail کلون AE1+AE3 برند Patho Sage
Specification:
Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratin’s, which include CK1, CK3, CK4, CK5, CK6, CK8, CK10, CK14, CK15, CK16, and CK19. AE1/AE3 is a broad-spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors (e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.
Immunogen:
Human epidermal keratin
Presentation:
Bioreactor Concentrate with 0.05% sodium azide, the ready-to-use antibody is diluted in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی CD15 کلون MMA برند Patho Sage
Specification:
CD15 recognizes a human myelomonocytic antigen. The structure recognized by CD15 antibodies is lacto-N-fucopentose III. The CD15 antigen is present on greater than 95% of mature peripheral blood eosinophils and neutrophils and is present at low density on circulating monocytes. In lymphoid tissue, CD15 reacts with Reed-Sternberg cells of Hodgkin`s disease and with granulocytes. However, CD15 reacts with few tissue macrophages and does not react with dendritic cells.
Immunogen:
U-937 histiocytic cell line
Presentation:
Bioreactor Concentrate with 0.05% Azide.
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی S100 Protein کلون 4C4.9 برند Patho Sage
Specification:
S-100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, as well as in glial cells. Neoplasms derived from these cells also express S-100 protein, albeit non-uniformly. A large number of well differentiated tumors of the salivary gland, adipose and cartilaginous tissue, and Schwann cell derived tumors express S-100 protein. Almost all malignant melanomas and cases of histiocytosis x are positive for S-100 protein. Despite the fact that S-100 protein is an ubiquitous substance, its demonstration is of great value in the identification of several neoplasms, particularly melanomas.
Immunogen:
Purified bovine brain S100 protein
Presentation:
Bioreactor Concentrate with 0.05% Azide, the ready-to-use antibody is diluted in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی Cytokeratin کلون 34BE12 برند Patho Sage
Specification:
Anti-Cytokeratin, 34betaE12 is an antibody to high molecular weight cytokeratin that reacts with all squamous and ductal epithelium and stains carcinomas. This antibody recognizes cytokeratins 1,5,10, and 14 that are found in complex epithelia. Anti-Cytokeratin, 34beta E12 shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules or endometrial glands; there has been no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors and neuroendocrine tumors are unreactive with this antibody. Anti-Cytokeratin, 34beta E12 does label myopeithelial cells and has been shown to be useful in distinguishing prostatic adenocarcinoma from hyperplasia of the prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.
Immunogen:
Solubilized keratin extract from human stratum corneum
Presentation:
Bioreactor Concentrate with 0.05% Azide, the ready-to-use antibody is diluted in Tris Buffer, pH 7.3-7.7, with
1% BSA and <0.1% Sodium Azide -
آنتی بادیهای ایمونوهیستوشیمیآنتی بادی CD138 کلون B-A38 برند Patho Sage
Specification:
B-A38, anti CD138, recognizes the 30.5 kD syndecan-1 proteoglycan expressed on human plasma cells, endothelial cells and fibroblasts. It is expressed on epithelial and endothelial cells and on cells of B lymphocyte lineage at discrete differentiation stages. Syndecan-1 is expressed on precursor B cells, is lost before maturation and entry into the circulation, and is re expressed upon plasma cells. It detects also thymocytes, activated T-cells, basophils, natural killer cells, and monocytes. B-A38, anti CD138, detects 70-100% of multiple myeloma cells and B cell chronic lymphocytic leukemia.
Presentation:
1 ml of 0.1 mg of purified IgG in of 0.01 M sodium phosphate, 0.15M NaCl; pH 7.3,0.2 % BSA,0.09% sodium azide.
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آنتی بادیهای ایمونوهیستوشیمی
آنتی بادی BAP1 کلون C-4 برند Patho Sage
Specification:
Mutations within the BRCA1 gene, localized to chromosome 17q, are believed to account for approximately 45% of families with increased incidence of both early-onset breast cancer and ovarian cancer. The BRCA1 gene is expressed in numerous tissues, including breast and ovary, and encodes a predicted protein of 1,863 amino acids. This protein contains a RING domain near the N-terminus and appears to encode a tumor suppressor. BARD1 (BRCA1-associated RING domain protein 1) and BAP1 (BRCA1-associated protein 1) have both been shown to bind to the N-terminus of BRCA1 and are potential mediators of tumor suppression. BARD1 contains an N-terminal RING domain and three tandem ankyrin repeats. The C terminus of BARD1 contains a region with sequence homology to BRCA1, termed the BRCT domain. BAP1 is a ubiquitin hydrolase and has been shown to enhance BRCA1-mediated cell growth suppression.
Immunogen:
Amino acids 430-729 of BAP1 of human origin
Presentation:
Antibody in PBS with < 0.1% sodium azide and 0.1% gelatin
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی YAP کلون 63.7 برند Patho Sage
Specification:
The Yes-associated protein, otherwise known as YAP, is a 14-3-3 binding molecule that was originally recognized by virtue of its ability to bind to the SH3 domain of Yes. The binding of YAP to 14-3-3 requires the phosphorylation of a homologous serine residue (Ser 112) in the YAP 14-3-3 binding motif. The highly conserved and ubiquitously expressed 14-3-3 proteins regulate differentiation, cell cycle progression and apoptosis by binding intracellular phosphoproteins involved in signal transduction. YAP may link events at the plasma membrane and cytoskeleton to inhibition of transcription in the nucleus in a manner regulated by 14-3-3 proteins. YAP shares homology with the WW domain of TAZ, transcriptional co-activator with PDZ binding motif, which functions as a transcriptional co-activator by binding to the PPXY motif present in transcription factors. YAP is expressed at high levels in the lung, placenta, prostate, ovary and testis.
Immunogen:
Recombinant YAP of human origin
Presentation:
YAP1 in PBS with less than 0.1% sodium azide and 0.1% gelatin
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی Kappa Immunoglobulin Light Chain کلون L1C1 برند Patho Sage
Specification:
This antibody is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
Immunogen:
Human B-Lymphoma Cells
Presentation:
Bioreactor Concentrate with 0.05% Azide
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی Desmin کلون D33 برند Patho Sage
Specification:
Desmin also known as Human muscle, 53kD. Desmin is an intermediate filament protein of both smooth and striated muscles. Antibody to Desmin reacts with striated (skeletal and cardiac) as well as smooth muscle cells. In skeletal and cardiac muscles, the staining is confined to the Z-bands giving a characteristic striated appearance. Anti Desmin antibody is useful in indication of tumors of myogenic origin. It reacts with leiomyosarcomas (smooth muscle) as well as rhabdomyosarcomas (striated muscle).
Immunogen:
Human leiomyoma
Presentation:
Diluted supernatant in 0.15 M PBS with approximately 25 μg antibody/ml and 1% BSA and 0.1% sodium azide
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی Vimentin کلون V9 برند Patho Sage
Specification:
This antibody is designed for the specific localization of Vimentin in formalin-fixed, paraffin-embedded tissue sections. It is intended for in vitro diagnostic use. Vimentin is the major intermediate filament in a variety of mesenchymal or mesenchymally derived non-muscle cell types, including endothelial cells, all fibroblastic cells, macrophages, neuroblastomas, Sertoli cells, melanocytes, lymphocytes and ovarian granulosa cells. This antibody binds specifically to the 57 kD intermediate filament vimentin using immunoblotting. Vimentin is found in all types of sarcomas and lymphomas. Positive staining for vimentin is seen in most cells of fibrosarcomas, liposarcomas, malignant fibrous histocytomas, angiosarcomas, chondrosarcomas and lymphomas. When the vimentin antibody is used in combination with other antibodies as a panel, it can aid in the histological classification of normal and malignant tissues.
Presentation:
Mouse Monoclonal antibody in TBS, pH 7.6, containing 1% BSA and 0.09% sodium azide.
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آنتی بادیهای ایمونوهیستوشیمی
آنتی بادی Cytokeratin 20 کلون KS20.8 برند Patho Sage
Specification:
This antibody reacts primarily with gastric and intestinal epithelium, urothelium, and Merkel cells. Anti-Cytokeratin 20 is useful in the differentiation of specific types of simple epithelial cells of the urinary tract as well as normal and malignantly transformed epithelia. Studies have identified the presence of Cytokeratin 20 in adenocarcinomas of the colon, stomach, pancreas and biliary system. Additionally, mucinous ovarian tumors, transitional-cell and Merkel cell carcinomas have shown reactivity. In contrast, squamous cell carcinomas and adenocarcinomas of the breast, lung and endometrium, non-mucinous tumors of the ovary, and small cell carcinomas are non-reactive.
Presentation:
Anti-Cytokeratin 20 is a mouse monoclonal antibody from supernatant diluted in tris buffered saline, pH 7.3
7.7, with protein base, and preserved with sodium azide -
آنتی بادیهای ایمونوهیستوشیمیآنتی بادی Melanoma کلون HMB45 برند Patho Sage
Specification:
This antibody gives a qualitative assessment of malignant melanoma, which can be extremely difficult to diagnose. Metastatic amelanotic melanoma can often be confused with a variety of poorly diferentiated carcinomas, large cell lymphomas, and sarcomas. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. Melanoma antibody stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanocytes. Typically, a keratin negative, vimentin rich neoplasm, that immunoreacts with antibody to S-100 protein and this melanoma antibody is, with rare exception, a melanoma.
Immunogen:
Extract of pigmented melanoma metastases from lymph nodes
Presentation:
Bioreactor Concentrate with 0.05% Azide
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آنتی بادیهای ایمونوهیستوشیمی
آنتی بادی BCL6 کلون ZM22 برند Patho Sage
Specification:
Recognizes a protein of 95kDa, which is identified as Bcl-6. Antibody to bcl-6 is helpful in a number of diagnostic settings: (1) In the differential diagnosis of small B-cell lymphoma. Follicular lymphoma will show bcl-6 (and CD10) positivity whereas other small B-cell lymphomas are usually negative. (2) Bcl-6 is an important prognostic marker in diffuse large Bcell lymphomas(DLBCL), where CD10, bcl-6 and MUM1/IRF4 are used to identify germinal center and activated B-cell phenotypes.(3) Bcl-6 can be valuable in distinguishing classical Hodgkin lymphoma from nodular lymphocyte predominant Hodgkin lymphoma (NLPHL). The Reed Sternberg cells of classical Hodgkin lymphoma are bcl-6 negative whereas the large (“L&H”) cells of NLPHL are bcl-6 positive. In contrast, anti-Bcl-6 rarely stains mantle-cell lymphoma and MALT lymphoma.
Immunogen:
Recombinant human BCL-6 protein fragment (aa256-389) (exact sequence is proprietary)
Presentation:
Purified antibody diluted in Tris-HCI buffer containing stabilizing protein and <0.1% sodium Azide.
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی P53 کلون BP53-12 برند Patho Sage
Specification:
Recognizes a 53kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53 under denaturing and non-denaturing conditions. Its epitope maps within the N-terminus (aa 20-25) of p53 oncoprotein. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.
Immunogen:
Recombinant human wild type p53 protein
Presentation:
Bioreactor Concentrate with 0.05% Azide
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی CD117 (c-kit) کلون YR145 برند Patho Sage
Specification:
CD117, c-kit is a tyrosine kinase receptor found on interstitial cells of Cajal, germ cells, bone marrow stem cells, melanocytes, breast epithelium and mast cells. This receptor is found on a wide variety of tumor cells (follicular and papillary carcinoma of thyroid, adenocarcinomas from endometrium, lung, ovary, pancreas, breast, malignant melanoma, endodermal sinus tumor, and small cell carcinoma) but has been particularly useful in differentiating gastrointestinal stromal tumors from Kaposi’s sarcoma, and tumors of smooth muscle origin.
Immunogen:
A synthetic peptide from C-terminus of human CD117 / c-kit protein
Presentation:
Purified antibody from tissue culture supernatant with 0.2% BSA and 15mM sodium azide