DESCRIPTION AND APPLICATIONS: Sheep anti Human fibrinogen antibody recognizes human fibrinogen, a complex ~340kDa heterohexameric (ditrimeric) glycoprotein consisting of 3 pairs of α, β and γ chains linked by a series of 29 disulphide bonds. The six chains are arranged in such a way that all the N-Terminal ends adjoin to form a central E domain with two trimeric coiled coil structures connecting to outer D domains. Fibrinogen plays an important role in the coagulation process with the D and E domains interacting via the C Terminal ends of the α chains during fibrin clot cross-linking. Sheep anti human fibrinogen antibody shows minimal cross-reactivity with related serum proteins. Fibrinogen has been identified as a ferritin binding protein in the horse. Sheep anti human fibrinogen antibody has been successfully as a capture reagent for ferritin – anti ferritin IgG complexes in horse plasma to evaluate theantibody response to ferritin by ELISA
This antibody should be used in conjunction with a panel of antibodies to aid in the identification of fibrinogen in target tissue (e.g., in the diagnosis of renal or dermal pathologies).
The interpretation of the results should be always corroborated with those of external controls and integrated with the morphological and clinical aspects for the final diagnosis.
Anti-human Sheep Antibody to Fibrinogen. Fluorescein Conjugated Polyclonal antibody purified from serum and prepared in buffer with carrier protein and preservative for stabilization.
IMMUNOGEN: Human fibrinogen purified from plasma.