Name: Rabbit Anti-Human Bcl-2 Monoclonal Antibody Clone EP36
Description and applications: The Bcl-2 family of proteins regulates apoptosis by controlling mitochondrial permeability and release of cytochrome c. Bcl-2 is an anti-apoptotic protein that resides in the outer mitochondrial wall and inhibits release of cytochrome c. Over-expression of Bcl-2 has been shown to promote cell survival by suppressing apoptosis. It has been documented that bcl-2 becomes deregulated in tumor cells as a result of translocation into the immunoglobulin heavy-chain locus and is therefore activated in B cell malignancies. Bcl-2 antibody is useful in differentiation of follicular lymphoma from reactive follicular proliferation (bcl-2 negative). In addition, bcl-2 expression has been shown to be correlated with disease prognosis in breast cancer, prostate cancer, ovarian cancer, endometrial cancer and colon cancer. The sensitivity of this antibody in diagnosis of follicular lymphoma Nucleolar dot-like staining is frequently seen with this antibody and it should not interfere with the staining interpretation.
Composition: Antibody to Bcl-2 is affinity purified and diluted in 10 mM Phosphate buffered saline (PBS), pH 7.2 containing 1% bovine serum albumin (BSA) and 0.09% sodium azide (NaN3).
Immunogen: A synthetic peptide corresponding to residues between BH3 and BH4 of human Bcl-2 protein