Product Composition: 10mM EDTA pH8.0.
Intended Use: Product for in vitro diagnostic use
Recommendations for Use:
Before use: The EDTA solution is 10x concentrated and therefore before use it should be diluted in a 1:10 range with distilled or deionized water (one part EDTA buffer and 9 parts water).
-Cut and mount sections on charged slides or silane coated.
-Dewaxing (using xylene or substitute) and rehydrate the tissue sections thru decreasing series of alcohol. Then follow with the step 3 and 4.
-Unmasking: various alternative methods could be used to carry out the antigen retrieval
process using the diluted EDTA buffer
-Remove the sections and place them into the usual washing buffer (recommended TBS / TBS-Tween pH 7.5 – reference MAD-004077R)
-Proceed with the immunostaining protocol as usual.