.(Name: GATA3 Antibody (Clone L50-823
Description and applications: GATA3 (GATA binding protein 3 to DNA sequence [A/T]GATA[A/G]) is a zinc finger transcription factor and plays an important role in promoting and directing cell proliferation, development, and differentiation in many tissues and cell types. GATA3 expression is primarily seen in breast carcinoma and urothelial carcinoma and only rarely found in tumors from other organs, such as endometrial endometrioid adenocarcinoma or yolk sac tumors. In the lymphoid system GATA-3 gene expression promotes receptor T cells and induces the activation of T helper type 2 cells. GATA3 is expressed in all breast lobular carcinomas and 91% of invasive ductal carcinomas (grade I, 100%; grade II, 89% and grade III, 86%1-2). GATA3 expression seems to be lower in luminal B subtype breast carcinoma. It has also been reported that GATA3 expression is correlated with the status of ER, PR and Her2 in breast carcinoma. GATA3 expression is found in urothelial carcinoma, especially in invasive and high grade tumors. Therefore, anti-GATA3 can be used in a panel of antibodies for diagnosis of unknown primary carcinoma, when carcinomas of the breast or bladder are a possibility. Isolated studies have demonstrated the usefulness of GATA-3, together with T-bet marker to differentiate between lymphocytic colitis and celiac disease. In germ cell tumors glandular areas of endodermal and trophoblastic differentiation might be stained. GATA 3 is also positive in many tissues such as parathyroid or salivary gland tumors; mucinous and non-mucinous pancreatic adenocarcinomas, squamous cell carcinomas, basal cell carcinomas, chromophobe carcinomas of the kidney, mesotheliomas, poorly differentiated papillary or mucinous adenocarcinomas of the lung, carcinomas of the stomach, colon, prostate, endometrium, thyroid, serous and Brenner benign and borderline ovarian tumors.
Composition: anti-human GATA3 mouse monoclonal antibody purified from ascites. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.