Name: Mouse anti-human p57kip2 Monoclonal Antibody
Description and applications: p57Kip2 (or CDKN1C) is a potent tight-binding inhibitor of several G1 cyclin complexes, and is a negative regulator of cell proliferation. The gene encoding human p57Kip2 is located on chromosome 11p15.5, a region implicated in both sporadic cancers, Wilm’s tumor, and Beckwith-Wiedemann syndrome (BWS), a cancer syndrome, making it a tumor suppressor candidate. BWS is characterized by numerous growth abnormalities and an increased risk of childhood tumors. Several types of childhood tumors including Wilms’ tumor, adrenocortical carcinoma and rhabdomyosarcoma display a specific loss of maternal 11p15 alleles, suggesting that genomic imprinting plays an important part. This region also contains two other imprinted genes, insulin-like growth factor II (IGF-II) and H19, both of which seem to be implicated in adrenal neoplasms. The practical use of p57kip2 antibody resides in the differential diagnosis of complete hydatidiform mole (comprised solely of paternal DNA and consequently with lack of nuclear expression in the cytotrophoblast and villous stromal cells), the incomplete hydatidiform mole (triploid) and edematous abortion. Extravillous trophoblastic islets, maternal decidua and stromal cells of the villi serve as internal controls in all three entities. The syncytiotrophoblast is negative in all cases.
Composition: anti-human p57kip2 mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide
Intended use : Immunohistochemistry (IHC) on paraffin embedded tissues. Not tested on frozen tissues or Western-Blotting
Immunogen: Recombinant human p57Kip2 protein.