آنتی بادی TIA1 کلون 2G9A10F5 برند Patho Sage

Specification:

Originally, the 2G9 monoclonal antibody (mAb) was described as identifying a 15 kDa protein found in the cytoplasmic granules of cytotoxic T cells that might be part of a larger 40 kDa molecule, ubiquitously expressed, named p40-TIA-1 and often referred to as TIA-1 in the literature. Now, however, there is evidence that the 2G9 mAb identifies a 17 kDa cytoplasmic granule membrane protein named GMP-17 that has no similarity with p40-TIA-1. The GMP-17 antigen is a 165 amino acid protein with 4 transmembrane domains: but it is not a typical member of the four transmembrane superfamily. It is identical with previously identified cytotoxic granule proteins called NKG7 and GIG-1 – for GCSF induced gene protein 1 –, isolated from NK cells and granulocyte-colony stimulating factor-treated mononuclear cells, respectively.  The GMP-17 protein is also found in the granules of CD14+ monocytes and neutrophils. Conversely, GMP-17 is not expressed in B lymphocytes or B-cell lines. In humans, NKG7, the GMP-17 gene, is localized on chromosome 19q13-33. As the target cell-induced NK cell degranulation results in translocation of GMP-17 from granules to the plasma membrane, a possible role for GMP-17 in the formation of junctions between effector cells and target cells has been suggested. Furthermore, sequence homology with calcium channel proteins has suggested that it may regulate ion channels required for cytotoxic effector functions. The 2G9 mAb was evaluated during the 5th HLDA Workshop on Human Leucocyte Differentiation Antigens, in the section of monoclonal antibodies reactive with intracellular antigens.

Immunogen:

Human bone marrow malignant cells from a non- B, non-T acute leukemia

Presentation:

Liquid purified Ascites, purified with Protein G Chromatography with 15mM Sodium Azide