آنتی بادی PDX1 (EP139)

Name: PDX1 Monoclonal Antibody clone EP139

Description and applications: The homeobox 1 duodenal protein (PDX1) is a transcription factor that is required for the embryonic development of the pancreas and the maturation of the β-cells of the islets of Langerhans. Moreover, PDX1 activates somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2 gene transcription. The PDX1 protein is encoded by the PDX1 gene, located in the chromosomal region 13q12.2. Clinically, mutations in the PDX1 gene have been shown to cause pancreatic agenesis and hereditary maturity onset diabetes mellitus of the young (MODY); likewise, the PDX-1 factor plays a major role in the pathogenesis of non-insulindependent (type II) diabetes mellitus. The PDX1 protein is initially expressed in the intestinal region of the embryo and then in the primitive pancreatic epithelium, where it allows its proliferation, branching, and differentiation. In the adult, this transcription factor is selectively expressed in endocrine cells, such as pancreatic beta cells, duodenum’s Brunner’s gland cells, and endocrine cells of the stomach’s pyloric region. Within the pancreas, PDX1 can also be observed in a subset of centroacinar and pancreatic duct exocrine cells, as well as in the beta cells of the islets of Langerhans. Increased expression of PDX1 has been reported in pancreatic (both exocrine and neuroendocrine), colon, and prostate tumours, suggesting that PDX1 may act as a biomarker in patients with these malignant tumours. However, further studies are needed to prove their specificity.

Composition: Anti-human PDX1 rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide

Intended use: Immunohistochemistry (IHC) on paraffin embedded tissues. Not tested on frozen tissues or Western-Blotting