دسته بندی: بافرهای ایمونوهیستوشیمی
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ایمونو هیستوشیمیEDTA Buffer 10X pH8
Product Composition: 10mM EDTA pH8.0.
Intended Use: Product for in vitro diagnostic use
Recommendations for Use:
Before use: The EDTA solution is 10x concentrated and therefore before use it should be diluted in a 1:10 range with distilled or deionized water (one part EDTA buffer and 9 parts water).
-Cut and mount sections on charged slides or silane coated.-Dewaxing (using xylene or substitute) and rehydrate the tissue sections thru decreasing series of alcohol. Then follow with the step 3 and 4.
-Unmasking: various alternative methods could be used to carry out the antigen retrieval
process using the diluted EDTA buffer-Remove the sections and place them into the usual washing buffer (recommended TBS / TBS-Tween pH 7.5 – reference MAD-004077R)
-Proceed with the immunostaining protocol as usual.
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ایمونو هیستوشیمیTris-EDTA Buffer 10X pH9
Product Composition: TRIS BASE and EDTA pH 9.00
Intended Use: Product for in vitro diagnostic use
Recommendations for Use:
Before use: The TRIS EDTA solution is 10x concentrated and therefore it must be diluted in a
1:10 range with distilled or deionized water (one part TRIS EDTA buffer and 9 parts water).
1. – Cut and mount sections on either charged or silane coated slides.
2A. – In case of using an automated PT Module for simultaneous dewaxing, rehydrating and antigen (HIER) retrieval, follow the instructions enclosed in the equipment data sheet. Basically, fill the tanks with 1.5 litres of diluted, ready-to-use buffer solution, place the slides without dewaxing into the racks and then immerse them into the buffer solution. Program the PT Module for a cycle of antigen retrieval of 20min at 95ºC and then cool to room temperature. Then follow with the step 4.
2B. – In case of not using the PT Module for HIER continue with dewaxing (using xylene or substitute) and rehydration of the tissue sections through decreasing series of ethanol. Then follow with the step 3 and 4.3. – Unmasking: different alternative methods could be used to carry out the antigen retrieval process using the diluted TRIS EDTA buffer:
3.1 Unmasking in microwave: Place the sections in the diluted solution of TRIS EDTA (Coplin jar or other container with a volume as large as the laboratory considers) and bring the microwave oven to 700 watts (watt) for 10-15 minutes). Cool to room temperature.
3.2 Unmasking in pressure cooker: Fill the pressure cooker with 1-2 litres of diluted TRIS EDTA buffer. While hydrating the slides, heat the solution avoiding boiling. Then immerse the sections in the preheated buffer and close the pressure cooker. Wait until the pressure rises to the second notch and run from here 1-2 minutes (depending on the antigen unmasking). Remove and cool the pot (you can speed up the process placing the pot under running water). Open when there is no pressure and cool the slides inside of the pot for 20-30 minutes.
4. – Remove the sections and place them into the usual washing buffer (recommended TBS / TBS-Tween pH 7.5 – reference MAD-004077R)
5. – Proceed with the immunostaining protocol as usual -
ایمونو هیستوشیمیCitrate Buffer 10X PH6
Product Composition: Sodium- CITRATE pH6.0.
Intended Use: Product for in vitro diagnostic use
Recommendations for Use:
Before use: The CITRATE solution is 10x concentrated and therefore it must be diluted in a 1:10 range with distilled or deionized water (one part CITRATE buffer and 9 parts water).
1. – Cut and mount sections on either charged or silane coated slides.
2A. – In case of using an automated PT Module for simultaneous dewaxing, rehydrating and antigen (HIER) retrieval, follow the instructions enclosed in the equipment data sheet. Basically, fill the tanks with 1.5 litres of diluted, ready-to-use buffer solution, place the slides without dewaxing into the racks and then immerse them into the buffer solution. Program the PT Module for a cycle of antigen retrieval of 20min at 95ºC and then cool to room temperature. Then follow with the step 4.
2B. – In case of not using the PT Module for HIER continue with dewaxing (using xylene or substitute) and rehydration of the tissue sections through decreasing series of ethanol. Then follow with the step 3 and 4.3. – Unmasking: different alternative methods could be used to carry out the antigen retrieval
process using the diluted CITRATE buffer:
3.1 Unmasking in microwave: Place the sections in the diluted solution of CITRATE (Coplin jar or other container with a volume as large as the laboratory considers) and bring the microwave oven to 700 watts (watt) for 10-15 minutes). Cool to room temperature.
3.2 Unmasking in pressure cooker: Fill the pressure cooker with 1-2 litres of diluted CITRATE buffer. While hydrating the slides, heat the solution avoiding boiling. Then immerse the sections in the preheated buffer and close the pressure cooker. Wait until the pressure rises to the second notch and run from here 1-2 minutes (depending on the antigen unmasking). Remove and cool the pot (you can speed up the process placing the pot under running water). Open when there is no pressure and cool the slides inside of the pot for 20-30 minutes.
4. – Remove the sections and place them into the usual washing buffer (recommended TBS / TBS-Tween pH 7.5 – reference MAD-004077R)
5. – Proceed with the immunostaining protocol as usual. -
ایمونو هیستوشیمیTBS Tween 20 Buffer 10X
Product Composition: The composition of the 10x concentrated product is: Tris-HCl 500 mM, NaCl 3M, 0.5 % Tween 20, pH 7.5.
Recommendations for Use:
Before start using the product: This solution is 10x concentrated, so before its use it must be diluted
1:10 with double-distilled or deionized water (1 part of buffer and 9 parts of water). -
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ایمونو هیستوشیمیPBS BUFFER
Product Composition: 10 mM Sodium/Potassium Phosphate pH 7.4, 0.9% Sodium Chloride
Recommendations for Use:
Before start using the product: The solution is concentrated, so a dilution prior to its use is required. To do this, the content of each sachet is diluted in 1 L of double-distilled or deionized water.
1- Cut and mount the sections on silane-treated slides.
2- Deparaffinize and hydrate the tissue sections.
3- Retrieval: For the retrieval process, the appropriate antigen retrieval agent is used for each antibody (specified by the manufacturer).
4.- Remove sections and place them in PBS, pH 7.40.
5.- Proceed with the immunohistochemical staining.