دسته: ایمونو هیستوشیمی

Specification:

It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43. 70-90% if T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be malignant lymphoma, especially a low grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.

Immunogen:

Recombinant full-length human SPN protein

Presentation:

Purified antibody from bioreactor concentrates by protein A/G. Prepared in 10mM PBS with 0.05% BSA and 0.05% Azide.

Specification:

The WT1 gene, identified as a tumor suppressor gene located at 11p13, is involved in the development of Wilms’ tumor. The WT1 gene encodes a transcription factor with four DNA-binding zinc fingers at the C terminus. In vitro studies showed that WT1 suppresses or activates a number of genes, including those for PDGF-A chain, EGF receptor, CSF-1, IGF-II, IGF-I receptor, RAR-α, c-myc, bcl-2 and WT1 itself. Immunohistochemically, WT1 is detected in the nucleus of tumor cells of Wilms’ tumor and mesothelioma; therefore, WT1 has traditionally been used as a diagnostic marker for these tumors. Recent reports showed that other types of cancers, such as ovarian serous cancers and rhabdomyosarcomas also express WT1.

Presentation:

Tissue culture supernatant containing 15mM sodium azide

Specification:

Recognizes a protein of -55kDa, identified as SOX I 0. This MAb is highly specific and does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequencespecific DNA binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled,and desmoplastic subtypes. It is expressedby metastatic melanomas and nodal capsular nevusin sentinel lymph nodes, but notby other lymph node componentssuch asdendritic cells, which usually express S I 00protein. Commonly used melanoma markers, such as anti-HMB-45andanti-Melan-A, are poorly expressed in desmoplasticmelanomas while SOX-10 is moderately to strongly expressed in desmoplastic melanomas. SOX- 10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-I 0 expression is also observed in mast cells.

Immunogen:

Recombinant fragment (155 Amino residues between aa 100-300) of human SOX10 protein

Presentation:

Bioreactor Concentrate with 0.05% Azide

Specification:

Anti-Neurofilament antibody stains an antigen localized in several neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilament. Neurofilaments are also present in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.

Presentation:

Anti-Neurofilament is a mouse monoclonal antibody from supernatant diluted in tris buffered saline, pH 7.37.7, with protein base, and preserved with sodium azide

Specification:

Recognizes a protein of 140kDa, which is identified as the complement receptor 2 (CR2) or CD21. This protein is expressed strongly on mature B cells, follicular dendritic cells and weakly on immature thymocytes and t lymphocytes. In B-cells ontogeny, CD21 appears after the pre-B-stage, is maintained during peripheral B-Cell development and is lost upon terminal differentiation into plasma cells. CD21 expression is also gradually lost after stimulation of B cells in vitro. CD21 functions as receptor for C3d. C3dg and IC3b complement components, for EBV and for IFNalpha. CD21 binds to CD23 and associates with CD19, CD81, and Leu13 to form a large signal-transduction complex involved in B cell activation.

Immunogen:

Recombinant fragment (around aa142-240) of human CR2 (CD21) protein (exact sequence is proprietary)

Presentation:

Bioreactor Concentrate with 0.05% BSA and 0.05% Azide

Specification:

Keratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins exists in combination with keratin 8. It was reported that tissues from gastrointestinal tract are positive for both keratin 8 and 18 but do not contain keratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for keratin 18. Clone DC10 recognizes a variety of simple epithelia including glandular epithelium but not stratified squamous epithelia.

Immunogen:

Human breast cancer PMC 42 cells

Presentation:

Keratin 18 is a purified mouse monoclonal antibody, 0.2% BSA and 15mM sodium azide.