Category: IHC-Antibodies

Specification:

PAX8 antibody recognizes a protein of 62kDa, identified as PAX8. It is a member of the paired box (PAX) family of transcription factors. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas, and atypical thyroid adenomas. PAX8 is expressed in the thyroid (and associated carcinomas), non-ciliated mucosal cells of the fallopian tubes, and simple ovarian inclusion cysts, but normal ovarian surface epithelial cells. PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas. PAX8 antibody may be used as an additional immunohistochemical marker for renal epithelial tumors.

Immunogen:

Recombinant human PAX8 protein

Presentation:

Purified antibody from bioreactor concentrate

Specification:

β-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenin’s at adherens junctions (zonula adherens). Deletions in the cytoplasmic domain of E Cadherin which eliminate catenin binding also result in a loss of cell adhesion, indicating that this binding is essential for E Cadherin function. Although the α- and β-Catenin’s have been cloned, very little is known about their biochemical roles. However, a link between β-Catenin and colon cancer has been described. β-Catenin was found to co-immunoprecipitated with the APC tumor suppressor protein in human colorectal tumor cell lines, as well as in human kidney 293 cells. E-Cadherin, however, was not detectable in these complexes. Thus, the APC Catenin complex may be affecting the transmission of contact inhibition signals and/or the regulation of cell adhesion.

Immunogen:

Mouse β-Catenin aa. 571-781

Presentation:

0.05 mg/ml in Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Specification:

This recombinant rabbit monoclonal antibody recognizes a protein of 38kDa that is identified as synaptophysin. It is an N glycosylated integral membrane protein found in neurons and endocrine cells. Synaptophysin contains four transmembrane domains and may function as a gap junction-like channel. This antibody identifies normal neuroendocrine cells and neuroendocrine neoplasms. Diffuse, finely granular, cytoplasmic staining is observed, which probably correlates with the distribution of the antigen within neurosecretory vesicles. Synaptophysin is an independent, broad-range marker of neural and neuroendocrine differentiation.

Specification:

This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive marker for angiomyolipoma’s. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Immunogen:

Recombinant hMART-1 protein

Presentation:

Bioreactor Concentrate with 0.05% Azide

Specification:

Cytokeratins 8 & 18 can be found in most simple epithelium, e.g. thyroid, female breast, gastrointestinal tract, and respiratory tract. Adenocarcinomas and most non-keratinizing squamous carcinomas will stain but keratinizing squamous carcinomas will not. This antibody is used when attempting to demonstrate the presence of Paget cells; there is very little keratin 18 in the normal epidermis so this will only stain Paget cells. This approach facilitates the interpretation using immunostains and is more sensitive than mucin histochemistry.

Presentation:

Anti-Cytokeratin 8 & 18 is a cocktail of two mouse monoclonal antibodies from ascites diluted in tris buffered saline, pH 7.3-7.7, with protein base, and preserved with sodium azide

Specification:

This antibody recognizes proteins of 80-200kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This MAb does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+).

Immunogen:

Human colon carcinoma extract

Presentation:

Bioreactor Concentrate with 0.05% Azide

Specification:

CEA (carcinoembryonic antigen, CD66) consists of a heterogeneous family of related oncofetal 200 kD glycoproteins that is secreted into the glycocalyx surface of gastrointestinal cells. The staining of CEA is considered important because: 1. In breast carcinomas, CEA staining correlated well with clinical outcome, independent of histologic type of tumors (with or without metastases). 2. A CEA-staining primary tumor usually shows CEA elevations when there is disease recurrence or progression. 3. Before proceeding with extensive or repeated blood monitoring of CEA as an index of biological activity of diseases, it is important to find out if the substance is present in the original malignant lesion, preferably at the time of biopsy. 4. Excellent correlation between CEA staining of primary and metastatic tumors (including micrometastases to the regional lymph nodes) has been observed with respect to cervical, colonic and ovarian carcinomas. Usually CEA is demonstrated as a linear labeling of the apical poles of cells lining the glandular lumen and, occasionally, as weak staining near the apex of colonic epithelial cells. More uniform cytoplasmic staining is a feature of more aggressive tumors. CEA, however, should not be used as a marker of differentiation because many colon and lung tumors actually show increased staining with differentiation. Pancreatic carcinomas, testicular tumor, gall bladder neoplasms and granular cell Myo blastomas stain positive, whereas malignant tumors of brain, prostate, skin, lymphoreticular tissues, hepatocellular carcinomas, esophageal squamous cell carcinomas, and mesothelioma fail to stain for CEA.

Immunogen:

Human colon carcinoma extract

Presentation:

Bioreactor Concentrate with 0.05% Azide

 Specification:

The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Immunogen:

Recombinant Helicobacter pylori Catalase protein fragment (around aa323-445) (Exact sequence is proprietary)

Presentation:

Bioreactor Concentrate with 0.05% Azide