آنتی­ بادی Clusterin (EP181)

Name: Clusterin Antibody Clone EP181

Description and applications: This monoclonal antibody recognises clusterin, a heterodimeric glycoprotein of 70-80 kDa. Clusterin is highly expressed in various tissues including pancreas, tonsil, nasopharynx, uterine cervix, and epididymis; it is also secreted into different fluids. Human clusterin is encoded by a single copy gene located on chromosome 8p12 and 8p21 with nine exons and eight introns, spanning approximately 17 kb. Clusterin has two isoforms: one is secreted directly into different fluids, and the other is expressed in the cytoplasm and in the cell nucleus. Clusterin is involved in numerous biological processes including lipid transport, epithelial differentiation, cell-matrix interaction, cell aggregation, and regulation of apoptosis in hormone-dependent epithelia. Clusterin has recently been proposed as an immunohistochemical marker specific for follicular dendritic cell tumours, since it is not expressed in the rest of dendritic cell neoplasms (Langerhans cell histiocytosis and interdigitating dendritic cell tumours) and it is very useful when included in the panel comprising CD21, CD35, S-100 protein, CD1a, CD68, actin, desmin, and cytokeratin for the differential diagnosis of dendritic cell tumours. Clusterin gene expression has been demonstrated by microarray analysis in systemic (80-100% of cases) and cutaneous (40-60%) anaplastic lymphomas, in some large B-cell lymphomas (10%), and very sporadically in peripheral T lymphomas and nodular sclerosis Hodgkin lymphomas. Among non-hematopoietic neoplasms, clusterin overexpression has been detected in breast, colon, pancreas, and prostate carcinomas. Overexpression in prostate carcinomas inhibits neoplastic cells’ apoptotic death; therefore, a protective effect against cell death by apoptosis is associated to this protein.

Composition: Anti-human Clusterin rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: Synthetic peptide corresponding to residues from human clusterin.