Name: Rabbit anti-human Cyclin E1 Monoclonal Antibody clone EP1263
Description and applications: This antibody reacts with human protein cyclin E. E-type cyclins (cyclin E1 and cyclin E2) are expressed during the late G1 phase of the cell cycle and until the end of the S phase. Cyclin E activity is limited by the passage of the cells through the “R” restriction point, which marks a “point of no return” for cells going into division from a quiescent state (G0) or through G1 and S phases. Cyclin E expression is mainly regulated by gene transcription by members of the E2F transcription factor family and by degradation through the proteasome catabolic pathway. Cyclin E binds and activates Cdk2 kinase and, through phosphorylation of this kinase’s substrate, the socalled pocket proteins, the cyclin/Cdk2 complex regulates the entry into the S phase by initiating a cascade of events that allows the specific expression of S-phase genes. In addition, cyclin E plays a direct role in the initiation of DNA replication and in the control of genomic and centrosome stability. Overexpression of cyclin E shortens the G1 phase of the cell cycle, accelerating progression to the S phase. High levels of cyclin E expression have been associated with the initiation and progression of different human neoplasms, particularly breast cancer, leukaemias, and lymphomas, among others.
Composition: Anti-human Cyclin E1 rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide
Intended use: Immunohistochemistry (IHC) on paraffin embedded tissues. Not tested on frozen tissues or Western-Blotting
Immunogen: Recombinant protein corresponding to residues from human protein cyclin E.