Name : Cytokeratin 8/18 antibody clone B22.1/B23.1
Description and applications:Cytokeratins 8&18 can be found in most simple epithelium,e.g. thyroid,female breast, gastrointestinal tract, and respiratory tract. Adenocarcinomas and most non-keratinizing squamous carcinomas will stain, but keratinizing squamous carcinomas will not. This antibody is used when attempting to demonstrate the presence of Paget cells; there is very little keratin 18 in the normal epidermis so this will only stain Paget cells. This approach facilitates the interpretation using immunostains and is more sensitive than mucin histochemistry.
Composition:Anti-human Cytokeratin 8/18 cocktail of two mouse monoclonal antibodies purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide
Intended use : Immunohistochemistry (IHC) on paraffin embedded tissues. Not tested on frozen tissues or Western-Blotting