.(Name: Epithelial Specific Antigen (Clone Ber-EP4
Description and aplications: This antibody reacts with two glycoproteins of 34 and 49 kD molecular weight present on the surface and in the cytoplasm of all epithelial cells except the surface layer of the squamous epithelium, hepatocytes and parietal cells of the stomach. This antibody is specific to HEA125 equivalent. The antibody shows a broad pattern of reactivity with human epithelial tissues like simple epithelium, basal layer of the stratified squamous non-keratinized epithelia and epidermis. In contrast to other known anti-epithelial antibodies, this antibody does not mark the mesothelial cells and only a small number of mesotheliomas stained positively. This antibody does not react with nervous, glial, muscle, mesenchymal or lymphoid tissue. Due to the labile nature of the epitope in tissues fixed in buffered formalin and embedded in paraffin, negative staining results should be considered with caution.
Composition: anti-Ber-EP4 mouse monoclonal antibody obtained from supernatant culture and prediluted in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal.
Immunogen: MCF-7 human breast carcinoma cell line.