پروب فیش IGH-MYEOV Fusion/Translocation FISH Probe

INTRODUCTION: The MYEOV gene was originally isolated by the application of the NIH/3T3 tumorigenicity assay with DNA from a gastric carcinoma. The chromosomal region 11q13 is frequently associated with genetic rearrangements in a large number of human malignancies, including B-cell malignancies and overexpression of MYEOV is frequently observed in breast tumors and oral, esophageal squamous cell carcinomas and multiple myeloma. MYEOV is a putative oncogene, located 360kb away from CCND1, which is thought to be activated in the translocation by becoming closely associated with IGH enhancers. The mantle cell lymphoma (MCL) is a B-cell non-Hodgkin lymphoma with an aggressive clinical course. It is genetically characterized by t(11;14)(q13;q32) and is present in about 95% of MCL patients. By lower frequency, t(11;14) is also detectable in B-cell prolymphocytic leukemia, myelomas and chronic lymphocytic leukemia. In multiple myeloma (MM), t(11;14) is the most common translocation, detectable in about 15-20% of all MM patients by FISH.
INTENDED USE: The IGH-MYEOV Fusion/Translocation FISH probe kit is designed to detect rearrangements involving the human IGH locus and MYEOV gene located on chromosome bands 14q32.33 and 11q13.3, respectively.

PROBE DESCRIPTION:The IGH Probe of the IGH-MYEOV Fusion/Translocation FISH Probe Kit labeled with the fluorochrome CytoGreen covers the 5’ and the center sequences of the IGH locus; it also covers the 3’ part and the neighboring downstream region. The MYEOV Probe of the IGH-MYEOV Fusion/Translocation FISH Probe Kit labeled with the fluorochrome CytoOrange covers a chromosomal region which includes the entire MYEOV gene. The probe set is optimized to reveal translocations between the two regions.