INTRODUCTION: Rearrangements and abnormal expression of the FUS gene (also known as TLS, ALS6, ETM4, FUS1, POMP75 or HNRNPP2) have been described in the alveolar rhabdomyosarcoma, prostate carcinoma and other types of tumors such as myxoid liposarcoma, low-grade fibromyxoid sarcoma or some cases of Ewing’s sarcoma.
INTENDED USE: The FUS Break Apart FISH probe is designed to detect rearrangements in the FUS human gene located in the band of the chromosome 16p11.2. Aside from detecting breaks that can lead to the translocation of parts of the gene, to its inversion or to its fusion with other genes, the probe can also be used to identify other aberrations of FUS such as deletions or amplifications.
PROBE DESCRIPTION: The 5’ telomeric fragment of the FUS Break Apart FISH probe labeled with the fluorochrome CytoOrange covers the 5’ portion (beginning) of the FUS gene and some adjacent genomic sequences. The 3’ centromeric fragment of the FUS Break apart FISH probe labeled with CytoGreen covers the center and the 3’ part (end) as well as the distal sequences of the gene. Both probes are flanking sequences in the FUS gene, in which variable cut-off points have been observed.