INTRODUCTION: Rearrangements and abnormal expression of the CCND1 gene (also known as BCL1, PRAD1, U21B31 or D11S287E) have been described in several types of hematological malignancies such as follicular mantle lymphoma [t(11;14) CCND1/IGH] or some cases of diffuse large B-cell lymphoma. Although up to 15-20% of the malignant myelomas usually show Cyclin D nuclear expression, these cases do not have CCND1 gene translocation. On the other hand, there are cases of cyclin D1-negative mantle cell lymphoma and only the detection of the CCND1 gene translocation after a hybridization study can establish a diagnosis.
INTENDED USE: The CCND1 Break Apart FISH probe is designed to detect rearrangements in the CCND1 human gene located in the band of the chromosome 11q13.3. Aside from detecting breaks that can lead to translocation of parts of the gene, to its inversion or to its fusion with other genes, the probe can also be used to identify other aberrations of CCND1 such as deletions or amplifications.
PROBE DESCRIPTION: The 3’ telomeric fragment of the CCMD1 Break Apart FISH probe labeled with the fluorochrome CytoOrange covers some genomic sequences adjacent to the 3’ portion (beginning) of the CCND1. The 5’ centromeric fragment of the CCND1 Break apart FISH probe labeled with CytoGreen covers the sequences adjacent to the gene’s 3’ end. Both probes are flanking sequences of the CCND1 gene, in which variable cut-off points have been observed.