Name: Mouse Anti-OCT 3/4 Monoclonal Antibody
Description and aplications: OCT-3 (officially POU5F1, also known as OCT4, OCT 3/4, OTF3 or OTF4) is a nuclear transcription factor involved in the initiation, maintenance and differentiation of germ cells and stem cells during embryonic development. The antibody recognizes a protein of 18kDa molecular weight encoded by the gene POU5F1 located on chromosome 6p21.3 which is essential in the development of the blastocyst. Three isoforms of OCT-3-called OCT-3A, OCT-3B and OCT3B1 are generated by alternative splicing. Isoforms A and B1 are expressed only in germ cell tumors while OCT3B isoform may also be expressed in tumors of somatic origin. This antibody is useful for the identification of transcription factor OCT-3/4 present in normal tissues (embryonic testis) and neoplastic ones. Gonadoblastomas, intratubular germ cell tumors, testicular seminoma, ovarian dysgerminomas, extragonadal germinomas and embryonal carcinoma show nuclear positivity with different degrees of intensity. By contrast the yolk sac tumors, spermatocytic seminomas, choriocarcinoma, testicular and ovarian teratomas are negative. Its expression has also been demonstrated in several tumor lines of somatic origin and linked to poor prognosis
Composition: anti-OCT3/4 mouse monoclonal antibody obtained from supernatant culture and prediluted in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal.
Immunogen: amino acids 1-134 of Oct-3/4 of human origin