آنتی‌ بادی P62 (3/P62 LCK LIGAND)

Name: P62 Antibody clone LCK LIGAND 3/P62

Description and applications: This antibody recognises phosphotyrosine p62, a member of the c-src family of cytoplasmic kinases, which binds as a ligand to the SH2 domains of p56 (Lcr) in the absence of phosphotyrosine. p62/SQSTM1 is an adapter protein targeting the protein aggregates ubiquitinated by lysosomal degradation. p62/SQSTM1 is selectively degraded via the autophagic pathway. Mallory’s hyaline and intracellular hyaline bodies are associated with chronic non-neoplastic liver diseases (steatohepatitis, related or not to alcohol abuse; toxic or metabolic chronic cholestasis) and with neoplastic liver diseases such as hepatocellular carcinoma. These inclusions are related to and contain keratin aggregates, especially keratin 8, ubiquitin, heat shock proteins, and the stress adaptor protein p62, stabilized by bonds catalysed by transglutaminases. P62 binds to ubiquitin and acts as an adaptor to bind ubiquitinated proteins. Autophagy is an evolutionary highly conserved degradative mechanism affecting the components of the cytoplasm that contributes to homoeostasis by making organelle replacement possible. Unlike the ubiquitin-proteosome system, the autophagy mechanism occurs in multiple steps that include the formation of the phagophore, which traps proteins and organelles for its degradation, and the autophagosome, which, through mechanisms involving cytoskeleton’s microtubules, fuses with lysosomes to form autolysosomes, where the material is eventually degraded. This antibody is useful for the identification of p62, found in Mallory’s hyaline and intracellular hyaline bodies present in chronic liver diseases and hepatocellular carcinomas. It is also useful to detect p62 in autophagic vacuoles present in various neurodegenerative, neoplastic infectious, and inflammatory neuromuscular diseases.

Composition: Anti-human P62 mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide