.(Name: TRAF1 (TNF receptor-associated factor 1) Antibody (clone H3
Description and applications: This antibody recognises TRAF1, with a molecular mass of 52 kDa, codified by TRAF1 gene, located in chromosome 9q33.2. TRAF (TNF receptor-associated factor) constitutes a family of adapter proteins (TRAFs 1-6) that bind to different membrane receptors to initiate different signalling pathways related to cell death and survival through the activation of NF-κB and pro-mitogenic protein kinases. Tumour necrosis factor (TNF) activates different cellular signals through TNF-R1 and TNF-R2 receptors, belonging to the TNF receptor superfamily, which includes CD95 or FAS and CD40. TRAF1 and TRAF2 are TNF receptor-associated factor 1 and 2, respectively. Together, they form a heterodymeric complex associated with TNF-R2’sintracytoplasmic domain. A third member of the family, or TRAF-3 (also known as CD40bp, CRAF-1 or LAP1), has been identified; it associates with the intracytoplasmic domain of CD40. TRAF is the major signal transducer for TNF and IL- 1/TLR receptor superfamilies, playing an important role in innate and adaptive immunity. TRAF1 is expressed in cells latently infected with Epstein-Barr virus. It may be a useful indicator in EBVassociated nasopharyngeal carcinomas by predicting the induction of apoptosis secondary to chemotherapy.
Composition:Anti-human TRAF1 (TNF receptor-associated factor 1) mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
Immunogen:Synthetic peptide corresponding to aa 173-295 of the central region of human TRAF-1.