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Name: Flow Cytometry Antibody IgG2-FITC, Clone SAG2

Antibody IgG2-FITC is designed for flow cytometry use as a direct immunofluorescence reagent in the identification and enumeration of IgG2 expressing cells. IgG2-FITC reacts with the Fc region of human IgG2.

IgG is the most abundant immunoglobulin in human serum (approximately 80% of total). Soluble IgG (sIgG) antibodies are distributed equally between the intra- and extravascular pools.

There are four different IgG subclasses responsible of protection against microorganisms: IgG1 (67% overall), IgG2 (22% overall), IgG3 (7% overall) and IgG4 (4% overall). These molecules have different biological properties as distinct ability to fix complement proteins and interact with phagocytic cell receptors. The IgG1 and IgG3 subclasses are particularly good opsonins and mediators of antibodydependent cell-mediated cytotoxicity (ADCC).

sIgG antibodies are important activators of complement but not as efficient as sIgM antibodies. IgG3 is the most efficient complement fixing IgG subclass, while IgG1 is somewhat less efficient, and IgG2 is even less so. IgG4 is unable to bind C1q and so cannot fix complement at all in the classical pathway.

Only IgG antibodies can cross the mammalian placenta, and maternal IgG1, IgG3, and IgG4 molecules have been found to be crucial for conferring the passive immunity that protects the developing human fetus and newborn in the first few months of life. IgG2 crosses the placenta with much lower efficiency.

CYT-IGG2F should be used with BulkLysis^TM (CYT-BL) buffer that allows you to stain a great number of cells and analyze lowfrequency subsets, as IgG2+ memory B cells and plasma cells. If another lysing protocol is used, two pre-staining washing steps using PBS + 1% (m/v) BSA + 0,09% (m/v) sodium azide must be performed. This reagent must be used by flow cytometry qualified personnel.

REAGENT COMPOSITION

Purified monoclonal anti-IgG2 antibody (mAb) conjugated with fluorescein isothiocyanate (FITC), supplied in phosphate buffered saline with 0,09% (m/v) sodium azide and BSA 1% (m/v).

• Clone: SAG2.
• Isotype: Mouse IgG1.
• Amount per vial: 25 tests using 3µL of mAb to 106 cells.

Name: Flow Cytometry Antibody IgG2-PE, Clone SAG2

Antibody IgG2-PE is designed for flow cytometry use as a direct immunofluorescence reagent in the identification and enumeration of IgG2 expressing cells. IgG2-PE reacts with the Fc region of human IgG2.

IgG is the most abundant immunoglobulin in human serum (approximately 80% of total). Soluble IgG (sIgG) antibodies are distributed equally between the intra- and extravascular pools.

There are four different IgG subclasses responsible of protection against microorganisms: IgG1 (67% overall), IgG2 (22% overall), IgG3 (7% overall) and IgG4 (4% overall). These molecules have different biological properties as distinct ability to fix complement proteins and interact with phagocytic cell receptors. The IgG1 and IgG3 subclasses are particularly good opsonins and mediators of antibody dependent cell-mediated cytotoxicity (ADCC).

sIgG antibodies are important activators of complement but not as efficient as sIgM antibodies. IgG3 is the most efficient complement fixing IgG subclass, while IgG1 is somewhat less efficient, and IgG2 is even less so. IgG4 is unable to bind C1q and so cannot fix complement at all in the classical pathway.

Only IgG antibodies can cross the mammalian placenta, and maternal IgG1, IgG3, and IgG4 molecules have been found to be crucial for conferring the passive immunity that protects the developing human fetus and newborn in the first few months of life. IgG2 crosses the placenta with much lower efficiency.

CYT-IGG2PE should be used with Bulklysis^TM (CYT-BL) buffer that allows you to stain a great number of cells and analyze low frequency subsets, as IgG2+ memory B cells and plasma cells. If another lysing protocol is used, two pre-staining washing steps using PBS + 1% (m/v) BSA + 0,09% (m/v) sodium azide must be performed.

This reagent must be used by flow cytometry qualified personnel

REAGENT COMPOSITION

Purified monoclonal anti-IgG2 antibody (mAb) conjugated with R-phycoerythrin (PE), supplied in phosphate buffered saline with 0,09% (m/v) sodium azide and BSA 1% (m/v).

• Clone: SAG2.
• Isotype: Mouse IgG1.
• Amount per vial: 25 tests using 3µL of mAb to 106 cells.

Name: Flow Cytometry Antibody IgG3-FITC, Clone SAG3

Antibody IgG3-FITC is designed for flow cytometry use as a direct immunofluorescence reagent in the identification and enumeration of IgG3 expressing cells. IgG3-FITC reacts with the hinge region of the heavy chain of human IgG3.

IgG is the most abundant immunoglobulin in human serum (approximately 80% of total). Soluble IgG (sIgG) antibodies are distributed equally between the intra- and extravascular pools.

There are four different IgG subclasses responsible of protection against microorganisms: IgG1 (67% overall), IgG2 (22% overall), IgG3 (7% overall) and IgG4 (4% overall). These molecules have different biological properties as distinct ability to fix complement proteins and interact with phagocytic cell receptors. The IgG1 and IgG3 subclasses are particularly good opsonins and mediators of antibody dependent cell-mediated cytotoxicity (ADCC) (1).

sIgG antibodies are important activators of complement but not as efficient as sIgM antibodies. IgG3 is the most efficient complement fixing IgG subclass, while IgG1 is somewhat less efficient, and IgG2 is even less so. IgG4 is unable to bind C1q and so cannot fix complement at all in the classical pathway.

Only IgG antibodies can cross the mammalian placenta, and maternal IgG1, IgG3, and IgG4 molecules have been found to be crucial for conferring the passive immunity that protects the developing human fetus and newborn in the first few months of life. IgG2 crosses the placenta with much lower efficiency.

CYT-IGG3F should be used with BulkLysis^TM (CYT-BL) buffer that allows you to stain a great number of cells and analyze low frequency subsets, as IgG3+ memory B cells and plasma cells. If another lysing protocol is used, two pre-staining washing steps using PBS + 1% (m/v) BSA + 0,09% (m/v) sodium azide must be performed.

This reagent must be used by flow cytometry qualified personnel. This reagent must be used by flow cytometry qualified personnel

REAGENT COMPOSITION

Purified monoclonal anti-IgG3 antibody (mAb) conjugated with fluorescein isothiocyanate (FITC), supplied in phosphate buffered saline with 0,09% (m/v) sodium azide and BSA 1% (m/v).

• Clone: SAG3.
• Isotype: Mouse IgG1.
• Amount per vial: 25 tests using 3µL of mAb to 106 cells.

Name: Flow Cytometry Antibody IgM-APC, Clone SADA4

Antibody IgM-APC is designed for flow cytometry use as a direct immunofluorescence reagent in the identification and enumeration of IgM expressing cells.

Monomeric IgM is always the first form and isotype of Ig generated by naive B cells. Following its initial activation by antigen, the naive B cell proliferates and differentiates, and its progeny produce the pentameric secreted form of IgM. Thus, it is IgM antibodies that are expressed first in any primary immune response. IgM antibodies comprise only about 5–10% of normal serum Ig. Although the bulk of IgM is found in the blood, if vascular permeability has been increased in a local area by the release of vasoactive compounds during an inflammatory response, IgM antibodies can exit the blood and enter the tissues to reach sites of infection. Although the concentration of IgM antibodies in the external secretions is very low compared to that of IgA, secretory IgM antibodies do play an important role in mucosal humoral immunity.

CYT-IGMAP should be used with Bulklysis^TM (CYT-BL) buffer that allows you to stain a great number of cells and analyze low-frequency subsets, as IgM+ memory B cells and plasma cells. If another lysing protocol is used, two pre-staining washing steps using PBS+1% (m/v) BSA + ≤0.09% (m/v) sodium azide must be performed.  This reagent must be used by flow cytometry qualified personal. This reagent must be used by flow cytometry qualified personnel

REAGENT COMPOSITION

Purified monoclonal anti-IgM antibody (mAb) conjugated with Allophycocyanin (APC), supplied in phosphate buffered saline with <0,1% (m/v) sodium azide.

• Clone: SA-DA4
• Isotype: Mouse IgG1
• Amount per vial: sufficient volume for 25 tests using 5µl per 106 cells

Name: Flow Cytometry Antibody IgM-PerCP-Cyanine5.5, Clone MHM-88.

Antibody IgM-PerCP-Cyanine5.5 is designed for flow cytometry use as a direct immunofluorescence reagent in the identification and enumeration of IgM expressing cells.

Monomeric IgM is always the first form and isotype of Ig generated by naive B cells. Following its initial activation by antigen, the naive B cell proliferates and differentiates, and its progeny produce the pentameric secreted form of IgM. Thus, it is IgM antibodies that are expressed first in any primary immune response. IgM antibodies comprise only about 5–10% of normal serum Ig.

Although the bulk of IgM is found in the blood, if vascular permeability has been increased in a local area by the release of vasoactive compounds during an inflammatory response, IgM antibodies can exit the blood and enter the tissues to reach sites of infection. Although the concentration of IgM antibodies in the external secretions is very low compared to that of IgA, secretory IgM antibodies do play an important role in mucosal humoral immunity.

CYT-IGMC2 should be used with BulkLysis^TM (CYT-BL) buffer that allows you to stain a great number of cells and analyze low frequency subsets, as IgM+ memory B cells and plasma cells. If another lysing protocol is used, two pre-staining washing steps using PBS + 1% (m/v) BSA + 0,09% (m/v) sodium azide must be performed.

This reagent must be used by flow cytometry qualified personnel.

REAGENT COMPOSITION

Purified monoclonal anti-IgM antibody (mAb) conjugated with Peridin chlorophyll protein-Cyanine 5.5 (PerCP-Cyanine5.5), supplied in phosphate buffered saline with 0,09% (m/v) sodium azide and BSA 1% (m/v).

• Clone: MHM-88.
• Isotype: Mouse IgG1.
• Amount per vial: 25 tests using 3µL per 106 cells