دسته بندی: آنتی بادیهای ایمونوهیستوشیمی

Name: Rabbit anti-human Gross Cystic Disease Fluid Protein-15 (BRST-2/GCDFP-15) Monoclonal Antibody (Clone EP95).

Description and Applications: Gross cystic disease fluid protein (GCDFP-15), also called prolactininducible protein (PIP), is a single polypeptide chain with a versatile function in human reproductive and immunological systems. GCDFP-15 binds to CD4, exerts a potent inhibition on T lymphocyte apoptosis mediated by CD4/T-cell receptor (TCR) activation, and carries a fibronectinspecific aspartyl protease activity. It is up regulated by prolactin and androgens, while it is down regulated by estrogen. In normal adult tissues, GCDFP-15 expression was found in all apocrine, lacrimal, ceruminous, and Moll’s glands and in numerous serous cells of the submandibular, sublingual, and minor salivary glands. The serous cells of nasal and bronchial glands were also positive. It is used as a marker of apocrine differentiation. GCDFP-15 has been found in the cyst fluid of cystic breast disease and primary and metastatic breast cancer, and considered a highly specific marker for identification of breast cancer. GCDFP-15 expression has also been found in other cancer types including salivary glands, sweat glands, prostate, and lung.

COMPOSITION: Anti-human BRST-2/GCDFP-15 rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

IMMUNOGEN: A synthetic peptide corresponding to residues in human Gross Cystic Disease Fluid Protein15 (GCDFP-15) protein.

Name: Cytokeratin HMW Antibody clone 34BE12

Description and applications: The proteins generically known intermediate filaments measuring between 7 and 22 nm diameter (ie with a size between -5 to 7 nm like actin and 22 to 25 nm like tubulin), are part, together with the above mentioned, of the vertebrates cytoskeleton. This superfamily comprises six subfamilies of molecules with different tissue expression. Cytokeratins constitute two homology groups (I and II) and in humans are encoded by more than 49 different genes on chromosomes 17 (I) and 12 (II). The nomenclature coined in 1982 by Moll and Franke assigns ranges of 1 to 8 for type II cytokeratins (neutralor basic) and between 9 and 21 for type I (acidic). Normally, keratins are assembled in heterodimers I / II and are co-expressed in pairs specific to each tissue. This antibody recognizes keratins of 68, 58, 56.5 and 50 kD obtained from the skin stratum corneum. By immunohistochemistry, the antibody reacts with squamous epithelium, ductal epithelia and other similar structure. This antibody is used confirm carius types of tumors like breast, pancreatic, bile duct, salivary gland, prostate and transitional cell carcinoma.

Composition: anti-HMW-CK mouse monoclonal antibody obtained from supernatant culture and prediluted in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal.

Immunogen: Solubilized keratin extracted from human stratum corneum.

Name: Rabbit Anti-Human Bcl-2 Monoclonal Antibody Clone EP36

Description and applications: The Bcl-2 family of proteins regulates apoptosis by controlling mitochondrial permeability and release of cytochrome c. Bcl-2 is an anti-apoptotic protein that resides in the outer mitochondrial wall and inhibits release of cytochrome c. Over-expression of Bcl-2 has been shown to promote cell survival by suppressing apoptosis. It has been documented that bcl-2 becomes deregulated in tumor cells as a result of translocation into the immunoglobulin heavy-chain locus and is therefore activated in B cell malignancies. Bcl-2 antibody is useful in differentiation of follicular lymphoma from reactive follicular proliferation (bcl-2 negative). In addition, bcl-2 expression has been shown to be correlated with disease prognosis in breast cancer, prostate cancer, ovarian cancer, endometrial cancer and colon cancer. The sensitivity of this antibody in diagnosis of follicular lymphoma Nucleolar dot-like staining is frequently seen with this antibody and it should not interfere with the staining interpretation.

Composition: Antibody to Bcl-2 is affinity purified and diluted in 10 mM Phosphate buffered saline (PBS), pH 7.2 containing 1% bovine serum albumin (BSA) and 0.09% sodium azide (NaN3).

Immunogen: A synthetic peptide corresponding to residues between BH3 and BH4 of human Bcl-2 protein

(Name: Mouse anti-human Cytokeratin 19  Monoclonal Antibody (Clone BA17

Description and Applications: Cytokeratin 19 is a member of type I acidic subfamily of intermediate filaments. It is expressed in variou different human tissues except in liver. Keratin 19 is not expressed in hepatocytes, therefore, antibody to keratin 19 is useful in the identification of liver metastasis. It is expressed by the great majority of breast carcinomas and used as a marker for sentinel lymph node evaluation both in frozen tissues and formalin fixed, paraffin embedded samples.

COMPOSITION: Anti-human Cytokeratin 19 mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

IMMUNOGEN: Detergent soluble extract of human mammary epithelium.

.(Name: Mouse anti-human BCL-6 Monoclonal Antibody (Clone LN22

Description and applications: Bcl-6 proto-oncogene product (a Kruppel-type zinc-finger protein) is mainly expressed in normal germinal center B cells and related lymphomas. Bcl-6 is involved in chromosome rearrangements at 3q27 in non-Hodgkin’s lymphomas and Bcl-6 rearrangements have been detected in 33%-45% of diffuse large B cell lymphomas. Bcl-6 has been detected immunohistochemically in follicular lymphomas, diffuse large B cell lymphomas, Burkitt’s lymphomas and in nodular lymphocyte predominant Hodgkin lymphoma.

Composition: anti-human BCL-6 mouse monoclonal antibody purified from ascites. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: Recombinant BCL-6 protein.

(Name: Mouse anti-human Desmin Monoclonal Antibody (Clone D33

 Description and applications: Desmin is an intermediate filament protein of both smooth and striated muscles Antibody to desmin reacts with striated (skeletal and cardiac) as well as smooth muscle cells. In skeletal and cardiac muscles, the staining is confined to the Z-bands giving a characteristic striated appearance. Anti-desmin antibody is useful in identification of tumors of myogenic origin. It reacts with leiomyosarcomas (smooth muscle) as well as rhabdomyosarcomas (striated muscle). The antibody does not stain epithelial or myoepithelial cells, endometrial stromal tumors or ovarian cancer, lymphoid tissue, osteocytes, chondrocytes or mesothelial cells.

Composition: anti-human Desmin mouse monoclonal antibody purified from ascites. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: Desmin from human muscle.

.(Name: Rabbit anti-human DOG-1 Monoclonal Antibody (Clone SP31

Description and aplications: Dog1 is a cell surface protein of unknown function selectively expressed in gastrointestinal stromal tumors (GIST). Among GIST cases with KIT mutations the Dog1 antibody identified 11% more cases than c-Kit antibody . Dog1 identifies vast majority of both c-Kit negative and PDGFRA mutated GIST cases that may still benefit from imatinib mesylate (Gleevac), an inhibitor of the kit tyrosin kinase. In addition, Dog1 immunoreactivity is seen in fewer cases of mesenchymal, epithelial tumours and melanomas when compared with c-Kit. The use of this highly sensitive and specific novel marker will increase the accuracy of GIST diagnosis.

 Composition: anti-human DOG1 rabbit monoclonal antibody purified from ascites. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: Synthetic peptide of human DOG-1 protein.

.(Name: EGFR (Epidermal growth factor receptor) Antibody (Clone EP22

Description and applications: The epidermal growth factor receptor (EGFR) is a transmembrane glycoprotein of 170 kDa with tyrosine-kinase activity after binding to the epidermal growth factor (EGF) and is involved in cell growth and differentiation. Tyrosine(Tyr) residues Tyr 1068, Tyr 1148 and Tyr 1173 located at the carboxy (C) terminal portion of the EGFR, are the places of autophosphorylation upon binding with the EGF. Determining the level of expression of EGFR is necessary to establish a specific treatment with monoclonal antibodies (cetuximab). The EGFR is overexpressed in some tumors of the colon, esophagus, stomach, breast, CNS, bladder, lung, head and neck, uterine cervix, vulva, ovary and endometrium. EGFR is expressed in a high percentage of metastases of squamous cell carcinomas of different locations and therefore its expression has been considered as an indicator of poor prognosis in these tumors. The antibody detects both EGFR phosphorylated on Tyr1068 of the mature human isoform 1 (corresponding to Y1092 from the precursor form P00533-1/p170), and also unphosphorylated EGFR.

Composition: anti-human EGFR rabbit monoclonal antibody purified by protein affinity and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: A synthetic phoshopeptide corresponding to residues Tyr1068 of human EGFR was used as immunogen.

Name: Rabbit Anti-Human Estrogen Receptor (ER) Monoclonal Antibody clone SP1

Description and aplications: This rabbit monoclonal antibody (clone SP1) specifically reacts with the human alpha isoform of the estrogen receptors not having been tested in other species as mouse, rat and hamsters were it shows reactivity.This antibody is useful for immunohistochemical identification of estrogen receptor in normal and neoplastic cells estrogen dependent. However, the description of various types of benign and malignant neoplasms positive for estrogen receptors as meningiomas, lung and stomach adenocarcinoma, and above all in breast cancer, the amount of estrogen receptors expression in the tissue is an important parameter in predicting the prognosis of the disease and its response to endocrine therapy.

Presentation: ready-to-use

Composition: anti-ER rabbit monoclonal antibody obtained from supernatant culture and prediluted in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal.

Immunogen: Synthetic peptide derived from the C-terminus of human estrogen receptor alpha

Species reactivity: In vitro diagnostics in humans. Not tested in other species

.(Name: Factor XIIIa (FXIIIa) Antibody (Clone EP292

Description and applications: Factor XIII (plasma transglutaminase, fibrin stabilizing factor) is a glycoprotein that circulates in blood as a tetramer, consisting of two A and two B subunits. Subunit A of factor XIII is an un-glycosylated 730-residue peptide with a molecular mass of 83 kD. It is the last enzyme generated in the blood coagulation cascade and is the zymogen for fibrinoligase, a transglutaminase that forms intramolecular gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules and thus stabilizes blood clots.

Factor XIII A is present in plasma, platelets, and monocytes, as well as macrophages and bone marrow precursors of these cell types and selective expression in subsets of stromal inflammatory cells. Factor XIII A is a marker for alternatively activated macrophages, while absence of Factor XIII A in monocyte-derived

macrophages is an indicator of their activated state. In addition, Factor XIII A is useful in distinguishing malignant fibrous histiocytoma (positive) from soft tissue tumor (negative).

Composition: anti-human FXIIIa rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: A synthetic peptide corresponding to residues of human Factor XIII A protein.