Category: Flowcytometry Lysis

Annexin V Binding Buffer

Company: BioLegend

Catalog : 422201

Size : 100 mL

:Description

Annexin V Binding Buffer has been formulated for flow cytometric labeling of apoptotic cells with Annexin V reagents. The buffer is provided as a ready-to-use solution.

Cell Staining Buffer

Company: BioLegend

Catalog Number : 420201

Size : 500 mL

Storage & Handling : Store between 2°C and 8°C

Description :

Cell Staining Buffer is an antibody diluent and cell wash buffer optimized for use in immunofluorescent staining of viable or fixed single cell suspensions. Cell Staining Buffer contains bovine calf serum as a protein carrier to reduce nonspecific binding of antibodies and fluorochrome reagents to target cells. It also contains a metabolic inhibitor, sodium azide (NaN ), to inhibit patching and capping of cell surface antigens. To prevent interference in biotin/avidin indirect staining protocols, Cell Staining Buffer is formulated without biotin.

Intracellular Staining Permeabilization Wash Buffer (10X)

Company: BioLegend

Catalog : 421002

Size : 100 mL

:Description

ntracellular Staining Permeabilization Wash Buffer is useful for intracellular staining procedures, e.g., in preparation of cells for staining intracellular cytokines or other proteins. Intracellular Staining ermeabilization Wash Buffer is used to permeabilize cells following fixation with Intracellular Staining Fixation Buffer (Cat. No. 420801). It is supplied as a 10X solution and should be diluted in deionized water prior to use. Intracellular Staining Permeabilization Wash Buffer has been formulated to have minimal effects on cells, reduce non-specific staining and enhance the signal to noise ratio. It can be used for antibody dilutions and cell washing during intracellular staining.

Propidium Iodide Solution

Company: BioLegend

Catalog : 421301

Size : 2 mL

Description :

Propidium iodide (PI) is a fluorescent dye that binds to DNA. When excited by 488nm laser light, it can be detected with in the PE/Texas Red® channel with a bandpass filter 610/10. It is commonly used in evaluation of cell viability or DNA content in cell cycle analysis by flow cytometry.

RBC Lysis Buffer (10X)

Company: BioLegend

Catalog : 420301

Size : 100 mL

:Description

Red Blood Cell (RBC) Lysis Buffer has been designed, formulated, and tested to ensure optimal lysis of RBCs in single cell suspensions with minimal effects on leukocytes. RBC Lysis Buffer is supplied as a 10X solution containing ammonium chloride, potassium carbonate, and EDTA, and should be diluted in deionized water prior to use. Nucleated RBCs are not effectively lysed with ammonium chloride.