دسته بندی: آنتی بادیهای ایمونوهیستوشیمی

(Name: Rabbit anti-human FSH (Follicle Stimulating Hormone) Monoclonal Antibody (Clone EP257

Description and applications: The pituitary glycoprotein hormone family includes follicle-stimulating  hormone, luteinizing hormone, chorionic gonadotropin, and thyroid-stimulating hormone. All of these glycoproteins consist of an identical alpha subunit and a hormone-specific beta subunit. FSH beta is the beta subunit of folliclestimulating hormone. In conjunction with luteinizing hormone, follicle-stimulating hormone induces egg and sperm production. The FSH antibody specifically labels Gonadotrophs in pituitatry. It may be useful in the classification of pituitary tumors.

Composition: Anti-human FSH (Follicle Stimulating Hormone) rabbit monoclonal antibody purified from . serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: A synthetic peptide corresponding to residues of human FSH (beta subunit) protein.

.(Name: Galectin 3  Antibody (Clone B2C10

Description and applications: The antibody recognizes a 30 kDa protein member of the family of the beta-galactosidase-binding lctin involved in cell growth, adhesion, inflammation and apoptosis processes. Galectin-3 is expressed in normal inflammatory cells (granulocytes) in vascular endothelium and in primary and metastatic malignancies of the colon, liver, breast, tongue and thyroid. This antibody is useful for differentiating between benign (adenomas) and thyroid malignancies. It may also be useful for the recognition of minimally invasive thyroid lesions. The combined use of Galectin-3 and HBME-1 may be helpful in the differential diagnosis between adenomas and oncocytic thyroid carcinomas. The sensitivity of galectin-3 to detect oncocytic carcinomas and the oncocytic variant of papillary carcinoma is 95%. The medullary carcinomas and normal thyroid follicles do not express galectin-3.

Composition: anti-Galectin 3 mouse monoclonal antibody obtained from supernatant culture and prediluted in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal.

Immunogen: Recombinant protein of the full length od the human Galectin-3 protein.

.(Name: GATA3 Antibody (Clone L50-823

Description and applications: GATA3 (GATA binding protein 3 to DNA sequence [A/T]GATA[A/G]) is a zinc finger transcription factor and plays an important role in promoting and directing cell proliferation, development, and differentiation in many tissues and cell types. GATA3 expression is primarily seen in breast carcinoma and urothelial carcinoma and only rarely found in tumors from other organs, such as endometrial endometrioid adenocarcinoma or yolk sac tumors. In the lymphoid system GATA-3 gene expression promotes receptor T cells and induces the activation of T helper type 2 cells. GATA3 is expressed in all breast lobular carcinomas and 91% of invasive ductal carcinomas (grade I, 100%; grade II, 89% and grade III, 86%1-2). GATA3 expression seems to be lower in luminal B subtype breast carcinoma. It has also been reported that GATA3 expression is correlated with the status of ER, PR and Her2 in breast carcinoma. GATA3 expression is found in urothelial carcinoma, especially in invasive and high grade tumors. Therefore, anti-GATA3 can be used in a panel of antibodies for diagnosis of unknown primary carcinoma, when carcinomas of the breast or bladder are a possibility. Isolated studies have demonstrated the usefulness of GATA-3, together with T-bet marker to differentiate between lymphocytic colitis and celiac disease. In germ cell tumors glandular areas of endodermal and trophoblastic differentiation might be stained. GATA 3 is also positive in many tissues such as parathyroid or salivary gland tumors; mucinous and non-mucinous pancreatic adenocarcinomas, squamous cell carcinomas, basal cell carcinomas, chromophobe carcinomas of the kidney, mesotheliomas, poorly differentiated papillary or mucinous adenocarcinomas of the lung, carcinomas of the stomach, colon, prostate, endometrium, thyroid, serous and Brenner benign and borderline ovarian tumors.

Composition: anti-human GATA3 mouse monoclonal antibody purified from ascites. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

.(Name: Glycophorin A  Antibody (Clone JC159

Description and aplications: This antibody reacts with a resistant paraffin-embedded and formol fixed epitope located in the extracellular domain of the protein, probably between amino acids 27 and 40. Therefore, this antibody recognizes normal erythroid cells in all stage of differentiation from erythroblasts to mature red blood cells. Once glycophorin A is at its maximum expression, the quantity in each erythroid cell remains constant and shows no change during maturation despite the decreasing size of the cell. The neoplastic erythroblast in most erythroleukemias are identified by Glycophorin A.

Composition: anti-Glycophorin A mouse monoclonal antibody obtained from supernatant culture and prediluted in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal.

Immunogen: membrane preparation from splenic hairy cell leukemia cells.

.(Name: Glypican 3 Antibody (Clone 1G12

Description and applications: Glypican‐3 (GPC3) is a member of the glypican family of glycosyl phosphatidylinositol‐anchored cell‐surface heparin sulfate proteoglycans. The 1G12 monoclonal antibody has been used to assess GPC3 expression in malignant and nonmalignant liver tissue samples and for enzyme‐linked immunosorbent assay (ELISA) for detection of GPC3 in the serum. Studies have shown that GPC3 is expressed at the protein level in most HCCs, but it is undetectable in normal liver and benign hepatic lesions, including dysplastic and cirrhotic nodules. In addition, GPC3 is significantly elevated in the serum of a large proportion of patients with HCC. Based on these results, it has been proposed that GPC3 could be a useful marker to differentiate between benign and malignant liver diseases. The GPC3 is expressed in the great majority of the gonadal yolk sac tumors. The endodermal component of endometrial or ovarian endometrioid carcinoma cases with also show intense cytoplasmic and / or membrane positivity. It is positive in sporadic cases of choriocarcinoma and presents focal positivity in embryonal carcinomas and immature teratomas; is not expressed in seminomas an intratubular germ cell neoplasia. Isolated cases spermatocytic seminomas show weak positivity. Other tumors of gonadal origin that can express GPC3 are endometrioid, clear cell, mucinous or serous carcinomas. 80% of placental site trophoblastic tumors and 100% of uterine placental site nodules are also positive. In other no gynecological tumors, GPC3 is expressed to 23% of lung carcinomas (55% squamous cell carcinomas and only 8% of adenocarcinomas) and 3% of renal cell carcinomas (chromophobe carcinomas). Along with other markers such as E-cadherin (negative) and β-catenin (positive nuclear and cytoplasmic), GPC3 is a useful marker in the diagnosis of pseudopapillary solid tumors of the pancreas and helps the differential diagnosis of neuroendocrine tumors. 34.7% of rhabdomyosarcomas, more frequent embryonal type, show positivity for this antibody while other types of sarcomas arenegative. In a recent study, 52% of liposarcomas and 29% of melanomas have been positive. Varying percentages of other tumors of epithelial and mesenchymal have shown variable positivity, demonstrating the lack of specificity of this antibody and the need to analyze the results in context with other antibodies staining.

Composition: anti-human Glypican 3 mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: Balb/C mice immunized with a single i.p. injection of 50 μg of a fragment containing the last 70 amino acids of the core protein

.(Name: Granzyme B Antibody (Clone GrB-7

Description and aplications: This antibody reacts with human Granzyme B. Granzyme B is a neutral serine protease which is stored in granules of specialized cytotoxic T lymphocytes (CD8) and NK cells. From mouse CD8 and NK cells vaious types of granzymes have been identifies and named alphabetically (A-G). They are involved in the apoptosis mediated by the cytotoxic lymphocytes. The exocytosis of granules containing granzyme in the cytoplasm of the target cells leads to DNA fragmentation and subsequent apoptosis. Granzyme B has also been located in EBV positive Reed Sternberg cells of Hodgkin’s disease.

Composition: anti-Granzyme B mouse monoclonal antibody obtained from supernatant culture and prediluted in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal.

Immunogen: Recombinant protein encoding the N-terminus of the mature granzyme B.

Name: Heat Shock Protein 70 (HSP70) Monoclonal Antibody clone W27

Description and applications: Heat shock proteins (HSP’s) or stress response proteins (SRP’s) are synthesized in variety of environmental and pathophysiological stressful conditions. Many HSP’s are involved in processes such as protein denaturation-renaturation, folding-unfolding, transporttranslocation, activation-inactivation, and secretion.HSP70 is found to be associated with steroid receptors, actin, p53, polyoma T antigen, nucleotides, and other unknown proteins. Also, HSP70 has been shown to be involved in protective roles against thermal stress, cytotoxic drugs, and other damaging conditions. This antibody recognizes both the constitutive (HSP73), and inducible (HSP72) forms of HSP70. Most normal cells show moderate cytoplasmic and nuclear immunostaining. Pancreatic islets, cardiac muscle cells and most CNS cells are weakly stained or negative. HSP70 in tumors is significantly increased in patients with cholangiocarcinoma. HSP70 is positive in the majority of the hepatocellular carcinoma. In oral squamous cell carcinomas HSP70 expression has been associated with increased survival compared with HSP70 negative tumors, suggesting that low HSP70 expression in epidermoid carcinomas require more aggressive treatment.

Composition: anti-human HSP70 rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide

Immunogen: HSP70 protein from HeLa cells

Species reactivity: In vitro diagnostics in humans. Not tested in other species

Name: Hepatocyte Specific Antigen (Hep Par 1) Antibody (Clone OCH1E5)

Description and applications: Anti-hepatocyte specific antigen, also known as anti-Hep-Par1, recognizes both benign and malignant liver-derived tissues including such tumors as hepatoblastoma, hepatocellular carcinoma (including its fibrolamellar variant), and hepatic adenoma. It recognizes both normal adult and fetal liver tissue. The typical pattern is a granular cytoplasmic staining. This antibody is useful in differentiating hepatocellular carcinomas with adenoid features from adenocarcinomas, either primary in the liver or metastatic lesions to the liver. In labeling hepatoblastoma, it is useful in differentiating this entity from other small round cell tumors. No reaction is usually seen with other human tumors with the exception of some gastrointestinal tumors. Occasionally it might be expressed by some tumors which include adrenal carcinomas, yolk sac tumors, adenocarcinomas of the colon, lung, ovarian carcinomas and adenocarcinomas of the endocervix.

Composition: anti-human HepPar 1 mouse monoclonal antibody purified from ascites. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

(Name: ZAP-70 Antibody (Clone 2F3.2

Description and applications:ZAP-70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene. In Western blotting of whole cell lysates of normal peripheral blood mononuclear cells, the antibody labels a band corresponding to ZAP-70. In Western blotting of whole cell lysates of CD19- positive purified leukemia cells from patients with lgunmutated and lg-mutated CLL, the antibody labels a band corresponding to ZAP-70 in the Ig-unmutated CLL samples, whereas no band is observed in the Igmutated CLL samples. In Western blotting of cell lysates of Jurkat cells (T-lymphoblastic cell line), the antibody labels a band of 70kDa protein. In Western blotting of cell lysates of A431 cells (carcinoma cell line), no band is observed. ZAP-70 protein is expressed in leukemic cells of approximately 25% ofchronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP-70 expression is an excellent surrogate marker for the distinction between the g-mutated (anti-ZAP-70 negative) and Ig-unmutated (anti-ZAP-70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP-70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.

Composition: Anti-human ZAP-70 mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: Recombinant ZAP-70 protein including residues 1-254 and encompassing SH2 domains of human ZAP-70.