.(Name: Mesothelial Cell Antibody (Clone HBME-1
Description and aplications: Antibody against HBME-1 stains the cytoplasmic membrane of normal pleural and peritoneal mesothelial cells and mesothelioma. Although both the membrane and the cytoplasm of mesothelioma cells stain positive with HBME-1, a thick membrane pattern turns out to be more useful for the diagnosis of malignant mesothelioma and it correlates with the presence of abundant “microvilli” in the surface membrane of these cells. There are other tissues (both malignant and normal) which are also positive with HBME-1, although immunostaining can be much weaker. Although HBME-1 positivity has been proven in 19 of 50 adenocarcinomas of different origin, the pattern of staining observed was cytoplasmic, although occasionally weak membrane staining also appeared. This antibody shows useful to distinguish malignant lesions of the thyroid (papillary and follicular) of benign lesions (goiter, adenomas, etc..). No cross reaction was observed with tissues such as kidney, liver, lung, ovary and pancreas. Storage and stability: up to 18 months; stored at 2- 8ºC. Do not freeze.
Composition: anti-HBME-1 mouse monoclonal antibody obtained from supernatant culture and prediluted in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal.