| REF | BU-006 |
|---|
Tris(hydroxymethyl)aminomethane (TRIS) is an organic compound commonly used as a component of buffer solutions. TRIS-buffered saline (TBS) has many applications because it is isotonic and non-toxic. It is used as a diluent and wash buffer in ELISA, Western blotting and general immunoassay procedures. In IHC it is used to reduce strong background staining or when using alkaline phosphatase conjugated antibodies. TBS is often used in dilution of secondary antibodies or streptavidinAP conjugates. Additives such as Tween20 allow additional functions.
