Specificity: The immunoglobulin molecules (Igs) are composed of two pairs of polypeptide chains, two heavy (IgH of 50-70 kD) and two light chains (IgL 23 kD), each of the light chains containing two successive domains: the first constant domain and the second a variable domain. Light chains are covalently linked to each correspondent heavy chain and are assembled through somatic rearrangement of the hypervariable region gene segment V (D) J. This process occurs during the early stages of the maturation of B lymphocytes, so that first (in the pro-B stage) the IgH chain rearrangement occurs to define the type of immunoglobulin that each cell will secrete and subsequently (in the pre -B stage) the IgL chain rearrangement occurs. In humans there are two IgL isotypes: Kappa ( ), encoded by a gene located on second chromosome and the Lambda ( ) with the coding gene located on chromosome 22. Each of the B-lymphocytes produces heavy chain immunoglobulins (IgG, IgM, IgA, IgD or IgE) and light chain immunoglobulins (IgL ) or (IgL ) but never both simultaneously. Under normal conditions, 60% of B lymphocytes produce light chain immunoglobulinas with
the rest represented by the chain. Therefore in all reactive lymphoid proliferation a mixed population of and chains positive cells is recognized, which is granted as a polyclonal expression.
As all malignant neoplasias, B cell lymphomas are clonal tumors originating from a transformed cell; hence, they are capable of producing only one type of immunoglobulins light chains, which is known as a monoclonal expression. Therefore the determination of the relationship between the two light chains of immunoglobulins in a cell population represents an essential tool for establishing the clonality in B cell lymphomas and neoplastic or reactive plasma cell dyscrasias. This probe is designed for the detection of mRNA of the kappa light chains ( ) through chromogenic in situ hybridization (CISH) techniques in tissues or cells fixed in 10% buffered formalin and paraffin embedded.
Sample Types: Sections of 4 microns thick mounted on special slides for immunohistochemistry and obtained from paraffinembedded
tissues, preferably fixed in buffered formalin.
Components and Reagents Included In The Kit:
Proteinase K Concentrated Solution (10 mg/mL) 20 tests
Digoxigenin-Labeled Human Ig-Kappa Probe 20 tests
Digoxigenin (HY-A.1) 20 tests
Peroxidase Blocking Reagent 20 tests
Primary Antibodies Amplifier Master 20 tests
Master Polymer Plus HRP 20 tests
DAB Substrate Buffer and DAB Chromogen Concentrate 20 tests
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