Name: Phospho-Histone H3 (PHH3) Antibody
Description and aplications: The phosphohistone H3 (phh3) is the phosphorylated form of nuclear histone H3, which together with the latter one forms the major protein component of the chromatin in eukaryotic cells. In mammalian cells the phosphohistone H3 is negligible during interphase, but reaches its maximum expression at mitosis, more precisely at the transition between the late G2 and M phases of the cell cycle. Immunohistochemical studies have shown that the PHH3 antibody specifically detects the nuclear histone H3 only when protein phosphorylation occurs at the level of serine 10 or serine 28. These studies have also shown that this phosphorylation during apoptosis does not occur on the histone H3, so that the presence of phh3 molecule can serve as a good marker for separating mitotic figures from the apoptotic bodies and nuclear karyorrhexis detritus and is likely to constitute a useful tool for tumor grading, treatment stratification and tumor follow-up especially in CNS, skin, lung, esophagus, breast, soft tissue, stromal gastrointestinal and gynecologic tumors. In fact, recent studies granted PHH3 with greater importance and relevance than Ki- 67 in identifying mitotic figures and a higher intra -and inter reproducibility in cases of melanoma.
Composition: anti- PHH3 rabbit polyclonal antibody obtained from supernatant culture and prediluted in a Tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal. The quantity of the active antibody was not determined.
Intended use : Immunohistochemistry (IHC) on paraffin embedded tissues. Not tested on frozen tissues or Western-Blotting