Name: Antibody PU-1
Description and applications: This antibody recognises a protein with a molecular mass of 42 kDa corresponding to PU.1 transcription factor, a member of the Ets family of transcription factors. It is required for the normal development and maturation of multiple hematopoietic lineages. PU.1 is a transcription factor restricted to the hematopoietic system, expressed in the myeloid series and in B cells but absent in mature T cells, the erythroid series, and in non-hematopoietic cells. Among the cells of the myeloid series, PU.1 is overexpressed in monocytes, histiocytes, and dendritic cells. Nodular lymphocyte-predominant Hodgkin lymphoma is consistently positive for PU.1, as opposed to classical Hodgkin lymphoma; therefore, PU.1, along with Pax-5, Oct-1, Oct-2, and BOB.1, is useful in establishing the differential diagnosis between both entities. PU.1 transcription factor and AML1, a DNAbinding subunit of the CBF transcription factor family, act as tumour suppressors in leukaemias. The function of PU.1 is down-regulated by AML1-ETO in myeloid leukaemias, while overexpression of PU.1 restores normal differentiation. High expression of PU.1 in follicular lymphomas gives them greater survival. PU.1 is a suitable marker to identify cutaneous neoplasms originating from histiocytes or from dendritic cells (reticulohistiocytomas, Langerhans cell histiocytosis, or juvenile xanthogranulomas).Dermatofibrosarcoma protuberans, dermatofibromas, fibrous papules, Spitz nevi, and melanomas are not stained with anti-PU.1.
Composition:Anti-human PU-1 rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide
Intended use : Immunohistochemistry (IHC) on paraffin embedded tissues. Not tested on frozen tissues or Western-Blotting