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Name: Flow Cytometry Antibody CD138-PECy5, Clone B-A38

• Antibody CD138-PECy5 is a monoclonal antibody (mAb) labelled with R-phycoerythrin-cyanine 5 (PECy5) designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of CD138 antigenexpressing cells.        

SUMMARY AND EXPLANATION

Human lymphocytes may be classified in three main populations according to their biological function and their cell surface antigen expression: T lymphocytes, B lymphocytes and natural killer cells (NK). B lymphocytes are the producers of antibodies and mediate humoral immunity particularly effective against toxins, whole bacteria, and free viruses. The CYT-138C4 mAb reacts with CD138, syndecan-1, a 20-22 kDa protein that binds to interstitial extracellular matrix molecules. CD138 is predominantly expressed on epithelial cells and B lymphocytes at specific stages in their differentiation: precursor B cells in the bone marrow and antibody-secreting cells, including plasma cells but not mature peripheral B cell.

REAGENT COMPOSITION

The purified monoclonal CD138 Antibody conjugated with R-phycoerythrin-cyanine 5 (PECy5) is supplied in phosphatebuffered saline (PBS) containing 0,09% sodium azide.

• Clone: B-A38.
• Isotype: IgG1.
• Amount per 1 mL vial: 200 tests (5 µL mAb per determination).
• Reagent is considered non-sterile.

Name: Flow Cytometry Antibody CD300e-APC, Clone UP-H2

• Antibody CD300e-APC is a monoclonal antibody (mAb) labelled with allophycocyanin (APC) designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of CD300e antigen-expressing cells.

SUMMARY AND EXPLANATION

FC is a powerful tool in analytical and quantitative characterization of cells which provides rapid and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. Flow cytometry is performed on cell suspension after incubating it with fluorescentlabelled antibodies directed against specific cellular proteins. Positive cells relative fluorescence intensity indicates the amount of antibody bonded to specific cell sites providing information about antigen expression. IREM2, also known as CD300e, is a 34 kDa integral membrane glycoprotein which is a member of the CMFRF35 subfamily. IREM2 is expressed on monocytes and myeloid dendritic cell lineage. During normal monocytic development, CD300e is expressed in the last stages of maturation. CD300e has been selected by EuroFlow consortium in AML (acute myeloid leukemia) antibody panel.

REAGENT COMPOSITION

The purified monoclonal CD300e antibody conjugated with allophycocyanin (APC) is supplied in phosphate-buffered saline (PBS) containing 0.1% sodium azide.

• Clone: UP-H2.
• Isotype: IgG1.
• Amount per vial: 100 tests (10ul/test).
• Reagent is considered non-sterile.

Name: Flow Cytometry Antibody IgA1-FITC, Clone SAA1

• Antibody IgA1-FITC is designed for flow cytometry use as a direct immunofluorescence reagent in the identification and enumeration of IgA1 expressing cells. IgA1-FITC reacts with the Fc portion of the heavy chain of human IgA1.

In humans, 85–90% of IgA antibodies are found in body secretions such us saliva, tears, breast milk, gastrointestinal mucous membranes, urogenital and respiratory tracts, and prostatic fluid, among others. The remaining fraction of 10–15% of IgA antibodies is found in blood, produced by B cells in the lymph nodes, bone marrow, and spleen. Two subclasses of human serum IgA exist. IgA1 tends to be monomeric and is present at a fivefold higher concentration than IgA2. Soluble IgA2 tends to be present more often in polymeric forms.

CYT-IGA1F should be used with BulkLysis^TM (CYT-BL) buffer that allows you to stain a great number of cells and analyze low-frequency subsets, as IgA1+ memory B cells and plasma cells. If another lysing protocol is used, two pre-staining washing steps using PBS + 1% (m/v) BSA + 0,09% (m/v) sodium azide must be performed.

This reagent must be used by flow cytometry qualified personnel.          

REAGENT COMPOSITION

Purified monoclonal anti-IgA1 antibody (mAb) conjugated with fluorescein isothiocyanate (FITC), supplied in phosphate buffered saline with 0,09% (m/v) sodium azide and BSA 1% (m/v).

• Clone: SAA1.
• Isotype: Mouse IgG1.
• Amount per vial: 25 tests using 3µL of mAb to 106 cells.

Name: Flow Cytometry Antibody IgA1-FITC, Clone SAA1

• Antibody IgA1-FITC is designed for flow cytometry use as a direct immunofluorescence reagent in the identification and enumeration of IgA1 expressing cells. IgA1-FITC reacts with the Fc portion of the heavy chain of human IgA1.

In humans, 85–90% of IgA antibodies are found in body secretions such us saliva, tears, breast milk, gastrointestinal mucous membranes, urogenital and respiratory tracts, and prostatic fluid, among others. The remaining fraction of 10–15% of IgA antibodies is found in blood, produced by B cells in the lymph nodes, bone marrow, and spleen. Two subclasses of human serum IgA exist. IgA1 tends to be monomeric and is present at a fivefold higher concentration than IgA2. Soluble IgA2 tends to be present more often in polymeric forms.

CYT-IGA1F should be used with BulkLysis^TM (CYT-BL) buffer that allows you to stain a great number of cells and analyze low-frequency subsets, as IgA1+ memory B cells and plasma cells. If another lysing protocol is used, two pre-staining washing steps using PBS + 1% (m/v) BSA + 0,09% (m/v) sodium azide must be performed.

This reagent must be used by flow cytometry qualified personnel.          

REAGENT COMPOSITION

Purified monoclonal anti-IgA1 antibody (mAb) conjugated with fluorescein isothiocyanate (FITC), supplied in phosphate buffered saline with 0,09% (m/v) sodium azide and BSA 1% (m/v).

• Clone: SAA1.
• Isotype: Mouse IgG1.
• Amount per vial: 25 tests using 3µL of mAb to 106 cells.

Name: Flow Cytometry Antibody IgA1-PE, Clone SAA1

• IgA1-PE is designed for flow cytometry use as a direct immunofluorescence reagent in the identification and enumeration of IgA1 expressing cells. IgA1-PE reacts with the Fc portion of the heavy chain of human IgA1.

In humans, 85–90% of IgA antibodies are found in body secretions such us saliva, tears, breast milk, gastrointestinal mucous membranes, urogenital and respiratory tracts and prostatic fluid, among others. The remaining fraction of 10–15% of IgA antibodies is found in blood, produced by B cells in the lymph nodes, bone marrow, and spleen. Two subclasses of human serum IgA exist. IgA1 tends to be monomeric and is present at a fivefold higher concentration than IgA2. Soluble IgA2 tends to be present more often in polymeric forms.

 CYT-IGA1PE should be used with BulkLysis^TM (CYT-BL) buffer that allows you to stain a great number of cells and analyze low frequency subsets, as IgA1+ memory B cells and plasma cells. If another lysing protocol is used, two pre-staining washing steps using PBS + 1% (m/v) BSA + 0,09% (m/v) sodium azide must be performed. This reagent must be used by flow cytometry qualified personnel.

REAGENT COMPOSITION

Purified monoclonal anti-IgA1 antibody (mAb) conjugated with R-phycoerythrin (PE), supplied in phosphate buffered saline with 0,09% (m/v) sodium azide and BSA 1% (m/v).

Clone: SAA1.

Isotype: Mouse IgG1.

Amount per vial: 25 tests using 3µL of mAb to 106 cells.

Name: Flow Cytometry Antibody IgA2-PE, Clone SAA2

• Antibody IgA2-PE is designed for flow cytometry use as a direct immunofluorescence reagent in the identification and enumeration of IgA2 expressing cells. IgA2-PE reacts with the Fc portion of the heavy chain of human IgA2.

In humans, 85–90% of IgA antibodies are found in body secretions such us saliva, tears, breast milk; gastrointestinal mucous membranes, urogenital and respiratory tracts and prostatic fluid, among others. The remaining fraction of 10–15% of IgA antibodies is found in blood, produced by B cells in the lymph nodes, bone marrow, and spleen. Two subclasses of human serum IgA exist. IgA1 tends to be monomeric and is present at a fivefold higher concentration than IgA2. Soluble IgA2 tends to be present more often in polymeric forms.

 CYT-IGA2PE should be used with BulkLysisTM (CYT-BL) buffer that allows you to stain a great number of cells and analyze low frequency subsets, as IgA2+ memory B cells and plasma cells. If another lysing protocol is used, two pre-staining washing steps using PBS + 1% (m/v) BSA + 0,09% (m/v) sodium azide must be performed. This reagent must be used by flow cytometry qualified personnel.

REAGENT COMPOSITION

Purified monoclonal anti-IgA2 antibody (mAb) conjugated with R-phycoerythrin (PE), supplied in phosphate buffered saline with 0,09% (m/v) sodium azide and BSA 1% (m/v).

Clone: SAA2.

Isotype: Mouse IgG1.

Amount per vial: 25 tests using 3µL of mAb to 106 cells

Name: Flow Cytometry Antibody IgD-FITC, Clone IADB6.

• Antibody IgD-FITC is designed for flow cytometry use as a direct immunofluorescence reagent in the identification and enumeration of IgD expressing cells. IgD-FITC reacts with the delta chain of human IgD. IgD is barely detectable in the blood (0,001% of total serum Ig) and is rarely secreted. It is observed only on the surface of immature, peripheral B cells that already express IgM. The expression of IgD is transient; upon activation of the B cell by the binding of antigen, the surface expression of mIgD, but not mIgM, is abrogated.

CYT-IGDF should be used with BulkLysis^TM (CYT-BL) buffer that allows you to stain a great number of cells and analyze low-frequency subsets, as IgD+ memory B cells and plasma cells. If another lysing protocol is used, two pre-staining washing steps using PBS + 1% (m/v) BSA + 0,09% (m/v) sodium azide must be performed.

REAGENT COMPOSITION

Purified monoclonal anti-IgD antibody (mAb) conjugated with fluorescein isothiocyante (FITC), supplied in phosphate buffered saline with 0,09% (m/v) sodium azide and BSA 1% (m/v).

• Clone: IADB6
• Isotype: Mouse IgG2a.
• Amount per vial: 25 tests using 3µL of mAb to 106 cells.

Name: Flow Cytometry Antibody IgD-PerCP-Cyanine5.5  , Clone IADB6.

• Antibody IgD-PerCP-Cyanine5.5 is designed for flow cytometry use as a direct immunofluorescence reagent in the identification and enumeration of IgD expressing cells. IgD-PerCP-Cyanine5.5 reacts with the delta chain of human IgD. IgD is barely detectable in the blood (0,001% of total serum Ig) and is rarely secreted. It is observed mostly on the surface of immature, peripheral B cells that already express IgM. The expression of IgD is transient; upon activation of the B cell by the binding of antigen, the surface expression of mIgD, but not mIgM, is abrogated.

CYT-IGDC should be used with BulkLysisTM (CYT-BL) buffer that allows you to stain a great number of cells and analyze low-frequency subsets, as IgD+ memory B cells and plasma cells. If another lysing protocol is used, two pre-staining washing steps using PBS + 1% (m/v) BSA + 0,09% (m/v) sodium azide must be performed.

REAGENT COMPOSITION

Purified monoclonal anti-IgD antibody (mAb) conjugated with Peridin chlorophyll protein-Cyanine 5.5 (PerCP-Cyanine5.5), supplied in phosphate buffered saline with 0,09% (m/v) sodium azide and BSA 1% (m/v).

• Clone: IADB6.
• Isotype: Mouse IgG2a.
• Amount per vial: 25 tests using 3µL of mAb to 106 cells