Category: IHC-Antibodies

Specification:

Recognizes a sialo glycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32kDa (p30/32). Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis. CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing’s sarcoma cells and primitive peripheral neuroectodermal tumors.

Immunogen:

Human acute lymphocytic leukemia T-cells

Presentation:

Bioreactor Concentrate with 0.05% Azide

Specification:

The catenin’s alpha, beta, and gamma are proteins which bind to the highly conserved, intracellular cytoplasmic tail of Ecadherin. Together, the catenin/cadherin complexes play an important role mediating cellular adhesion. Catenin, a 102 kDaprotein, was initially described as an E-cadherin-associated protein and has been shown to associate with other members of the cadherin family N-cadherin and P-cadherin. The 92 kDa catenin associates with the cytoplasmic portion  of E-cadherin which is necessary for the function of E-cadherin as an adhesion molecule. Catenin has also been found in complexes with the tumor suppressor protein APC. Catenin, also known as plakoglobin, is an 82 kDa protein that binds with catenin and Ncadherin. A related protein, p120, exhibits sequence homology with the catenins at four discreet domains. p120 not only serves as a substrate for Src but is also found in E-cadherin complexes containing catenins. p120 (15D2): sc-23872. Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast tissue showing membrane and cytoplasmic localization.

Presentation:

Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide 1 and 0.1% gelatin

Specification:

The LIM-only (LMO) proteins, LMO1 and LMO2, are nuclear factors that are characterized by a conserved LIM domain. The LIM domain consists of a cysteine-rich zinc-binding motif that is present in a variety of transcription factors, including the LIM homeobox (LHX) proteins expressed in the central nervous system and involved in cell differentiation. LMO1 and LMO2 are expressed in the adult CNS in a cell type-specific manner, where they are differentially regulated by neuronal activity and are involved in regulating the cellular differentiated phenotype of neurons. LMO2 lacks a specific DNA-binding homeobox domain but rather assembles into transcriptional regulatory complexes to mediate gene expression by interacting with the widely expressed nuclear LIM interactor (NLI). NLI, known also as CLIM-1, and the related protein CLIM2 facilitate the formation of heteromeric LIM complexes and also enhance the nuclear retention of LIM proteins. LMO2 and the related protein LMO4 are expressed in thymic precursor cells. LMO4 is also expressed in mature T cells, cranial neural crest cells, somite, dorsal limb bud mesenchyme, motor neurons, and Schwann cell progenitors.

Immunogen:

Recombinant LMO2 of human origin

Presentation:

Each vial contains 200 µg IgG1 in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Specification:

Recognizes a phosphor-protein of 45kDa, identified as MyoD1. The epitope of this MAb maps between amino acid 180-189 in the C-terminal of mouse MyoD1 protein. It does not cross react with Myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm’s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and in rhabdomyosarcomas, neuroblastomas, Ewing’s sarcomas and alveolar soft part sarcomas.

Immunogen:

Recombinant mouse MyoD1 protein

Presentation:

Bioreactor Concentrate with 0.05% Azide