Category: IHC-Antibodies

Bcl2 (B-cell lymphoma 2) plays an important role in regulation of apoptosis. It is expressed in different cells and tissues. Overexpression due to chromosomal translocation, gene amplification, increased gene transcription and/or altered post-translational processing is found in many cancer types. These include B-cell malignancies, such as B-cell lymphoma, follicular lymphoma (FL) and chronic lymphocytic leukemia (CLL), as well as some T-cell lymphomas, breast cancer, lung cancer, ovarian cancer, and prostat a cancer.

BOB.1 is a lymphoid-specific transcriptional coactivator, which interacts with the transcription factors Oct-1 andOct-2. BOB.1 is expressed in germinal center B-cells, mantle B-cells and plasma cells. It detects a variety of B-cell lymphomas and Hodgkin’s lymphoma.

Arginase (ARG) is a manganese metalloenzyme that is part of the urea cycle. It catalyses the conversion of L-arginine to L-ornithine and urea. ARG-1 and ARG-2 are the two isoforms of ARG. ARG-1 is a cytosolic enzyme expressed in liver. The ARG-1 antibody is a sensitive marker of hepatic differentiation and is useful in the detection of hepatocytes in normal tissue as well as granulocytes in peripheral blood. For the identification of hepatocellular carcinoma ARG-1 is a sensitive and specific marker.

Anti-human antibody for immunohistochemical use. The primary antibody is intended for qualitative detection of antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections. The antibody may be used manually or with any automated staining platform. Authorized and skilled personnel may only use the product. The clinical interpretation of any test results should be evaluated within the context of the patient’s medical history and other diagnostic laboratory test results. A qualified pathologist must perform evaluation.

Keratins are cytoplasmic intermediate filament proteins expressed by epithelial cells and their neoplasms. Cytokeratin 20 is a type I keratin mainly found in the gastric and intestinal mucosa. Antibody Cytokeratin 20 is useful in the detection of a variety of adenocarcinomas arising from epithelia, such as colorectal carcinomas, transitional-cell and Merkel cell carcinomas, gastric and pancreatic tumors. CK20 is often used in a panel with antibody Cytokeratin 7.

CD3 is a protein complex that is composed of five chains (gamma, delta, epsilon, zeta, eta) which associate with theT-cell receptor (TCR). CD3 is first expressed in the cytoplasm of developing T-cells, then as they mature it moves to the membrane. CD3e is a membrane protein that plays a role in TCR-CD3 complex assembly and signal transduction regulating T-cell proliferation, apoptosis and thymocyte development. CD3e immunostaining is only seen in T-cells. These predominantly occur in lymphatic organs such as thymus, lymph nodes and tonsils but are regularly also seen – at lower density – in many other tissue types. In malignant lymphomas, CD3 is a pan-T-cell lineage-restricted antigen, detected in 72-95% of the T-cell lymphomas. As B-cell lymphomas are mostly negative forCD3e, the antibody can be used to distinguish between T-cell and B-cell lymphomas.