پروب فیش IGH Break Apart FISH Probe

INTRODUCTION: Rearrangements involving the IGH locus (or immunoglobulin heavy locus) have been observed in many types of leukemias and lymphomas. It represents the prototype for translocations in B-cell non-Hodgkin lymphomas, in which it has been detected in up to 50% of the cases, whereas reciprocal and specific translocations with different chromosomal loci have been characterized and considered as pathognomonic for different histological subtypes.

INTENDED USE: The IGH Break Apart FISH probe is designed to detect rearrangements in the human IGH locus located in the chromosome band 14q32.33. Aside from detecting breaks that can lead to translocation of parts of the locus, to its inversion or to its fusion with other genes, the probe can also be used to identify other aberrations of IGH such as deletions or amplifications.

PROBE DESCRIPTION: The telomeric 5’ Fragment of the IGH Break Apart FISH probe labeled with the fluorochrome CytoOrange covers the 5’ portion and the center of the locus of the IGH gene, whereas the centromeric 3’ fragment of the IGH Break Apart FISH probe labeled with CytorGreen covers some distal sequences (3’ end) of the gene.Both loci are flanking sequences of the BCL2 gene, in which numerous cut-off points have been observed.