پروب فیش TFE3 Break Apart FISH Probe

INTRODUCTION: Rearrangements and abnormal expression of the TFE3 gene (also known as TFEA, RCCP2, RCCX1 or bHLHe33) have been described in a special subtype of renal cell carcinoma associated with Xp11.2 translocation, in alveolar sarcoma and other tumor types.

INTENDED USE: The TFE3 Break Apart FISH probe is designed to detect rearrangements in the TFE3 human gene located in the band of the chromosome Xp11.23. Aside from detecting breaks that can lead to translocation of parts of the gene, to its inversion or to its fusion with other genes, the probe can also be used to identify other aberrations of TFE3 such as deletions or amplifications.

PROBE DESCRIPTION: The 5’ centromeric fragment of the TFE3 Break Apart FISH probe labeled with the fluorochrome CytoOrange covers the 5’ portion (beginning) of the TFE3 gene and some adjacent genomic sequences. The 3’ telomeric fragment of the TFE3 Break apart FISH probe labeled with CytoGreen covers the 3’ part (end) and the adjacent and inferior sequences of the gene. Both probes are flanking sequences in the TFE3 gene, in which variable cut-off points have been observed.