Name: Mouse anti-human OCT-2 Monoclonal Antibody clone PT2
Description and aplications: Transcription factors Oct1 and OCT2 are located on chromosomal regions 1q22-q23 and 19 respectively, and belong to the POU family homeobox genes that bind DNA in monomeric and dimeric several configurations. Depending on the DNA sequence with which the dimers are joined, they are aligned in an accessible (MORE sequence) or inaccessible (PORE sequence) configuration for specific performance of the cofactor OBF1 of the B lymphocytes (Ocab, BOB1). OCT2 is an important element for the regulation of cellular and tissue specific transcription as well as transcription of numerous genes controllers. While Oct1 expression is an ubiquitous, OCT2 nuclear transcription factor expression is restricted to normal neurons of the central nervous system and B lymphocytes. This antibody recognizes the transcription factor Oct-2 of human, murine and rat origin and does not crossreact with other transcription factors. Oct-2 is expressed in normal B lymphocytes with greater intensity in the germinal center of lymphoid follicles. Small lymphocytes of the mantle areas have lower intensity and a small number of lymphocytes of the marginal and interfollicular areas also show intense nuclear staining. Since the expression of Oct-2 is related to the state of maturation of B cells, the antibody is primarily useful in the identification of B cell lymphomas originated in the germinal centre. In contrast to the L & H cells of lymphocyte-predominant Hodgkin’s disease (HD), the Reed-Sternberg (RS) cells of HD classical type are unable to transcribe immunoglobulins due to the absence of Oct-2. Therefore Oct-2 has been considered a new marker to identify and differentiate the L & H from HRS cells in Hodgkin’s disease. Immunostaining for Oct-2 is present intensively in nodular lymphocyte-predominant HD and with less intensity in the variants of the classic HD (HD rich in lymphocytes, HD mixed cellularity, HD nodular sclerosis) numerous cases being Oct-2 negative. In the nervous system it is considered that the expression of Oct-2 plays an important role in female sexual maturation in mammals regulated by the hypothalamus and the regulation of the latency period after infection with herpesvirus.
Composition: anti-human OCT2 mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide
Immunogen: full length Oct-2 of human origin
Species reactivity: In vitro diagnostics in humans. Not tested in other species
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