Category: IHC-Antibodies
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی Prostatic Acid Phosphatase (PASE/4LJ)
Name: Prostatic Acid Phosphatase Antibody Clone PASE/4LJ
Description and Applications: Anti-PSAP reacts with prostatic acid phosphatase in the glandular epithelium of normal and hyperplastic prostate, carcinoma of the prostate and metastatic cells of prostatic carcinoma. This marker may be helpful in pinpointing the site of origin in cases of metastatic carcinoma of the prostate, and is considered a more sensitive marker than PSA. However, it also offers less specificity. Nevertheless, PSAP complements PSA in the right clinical context.
Composition: Anti-human Prostatic Acid Phosphatase mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی Human IgD (Polyclonal)
Name: IgD Antibody (Polyclonal)
Description and applications: Anti-IgD antibody reacts with surface immunoglobulin IgD delta chains. This antibody is useful when identifying leukemias, plasmacytomas, and B-cell lineage derived lymphomas (in particular Marginal Zone Lymphoma). Cytoplasmic staining is easily identified on paraffin tissue. Surface immunoglobulin is dificult to demonstrate on paraffin sections but can be demonstrated on frozen sections.
Composition: anti-human IgD rabbit polyclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی Human IgG (E20-V)
Name: IgG Antibody Clone E20-V
Description and applications: The antibody labels the IgGs contained in the plasma cells and their precursors. In general, membrane bound immunoglobulins, extracellular immunoglobulins bound to connective tissue or blood vessels, and immunocomplexes can only be demonstrated in frozen tissues. Plasma cells may not
be intensely stained in frozen tissue since the immunoglobulins are distributed diffusely through the cytoplasm of these cells. This antibody is useful for identifying leukemias, plasmacytomas and B cells derived from Hodgkin lymphomas. Due to the restrictions on the expression of light and heavy chains in these diseases, demonstration of B-cell lymphomas is possible with clonal rearangements studies. Another application isComposition: Anti-human IgG rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی Human IgM (Polyclonal)
Name: IgM Antibody (Polyclonal)
Description and applications: Anti-IgM antibody reacts with surface immunoglobulin IgM mu chains. IgM is one of the predominant surface immunoglobulins on B-lymphocytes. This antibody is useful when identifying lymphomas, plasmacytomas, and B-cell lineage derived Hodgkin’s lymphomas. Due to the restricted expression of heavy and light chains in these diseases, demonstration of B-cell lymphomas is possible with clonal gene rearrangement studies.
Composition: Anti-human IgM rabbit polyclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی IgG4 (EP138)
Name: IgG4 Antibody Clone EP138
Description and applications: Human IgG4, one of four subclasses of IgG, contains a gamma 4 heavy chain and a hinge region that is shorter than that of IgG1. No allotypes have been detected on the heavy chains of IgG4. Its two primary effector functions are activating complements and binding to the FcgR of effector cells to initiate phagocytosis. Human IgG4 accounts for less than 6% of the total IgG serum level. Recent studies show that serum levels and immunohistochemistry staining with IgG4 antibody is a useful diagnosis marker for IgG4-related sclerosing diseases. A new concept of IgG4-related systemic disease (ISD) has been established recently. The ISD is characterized by elevated serum IgG4 levels and extensive IgG4+ plasma cell infiltrate in pancreas and/or in other organs, including peripancreatic tissue, bile duct, gallbladder, portal area of the liver, gastric mucosa, colonic mucosa, salivary glands, lymph nodes, and bone marrow. Immunohistochemistry analysis of IgG4 is useful for identifying ISD.
Composition: anti-human IgG4 rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی Immunoglobulin J chain (SP105)
Name: J Chain Immunoglobulin Antibody Clone SP105
Description and applications: J chain is a small glycopeptide and is structurally unrelated to heavy or light chains, but is synthesized by all plasma cells that secrete polymeric immunoglobulins. J chain is linked to IgA and IgM by disulfide bonds, however, it has been detected in IgG- and IgD-containing cells. J chains are present in a large proportion of the immunoglobulin-positive cells in the germinal centers of the tonsil and lymph node. B cells secrete J chain at an early stage of differentiation with the expression persisting in those cells destined to produce IgA or IgM. It has been shown that a significant proportion of mesangial immunoglobulin deposits of IgA nephropathy are dimeric and therefore positive for this antibody.
Composition: anti-human J Chain immunoglobulin rabbit monoclonal antibody purified and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی IMP 3 (EP286)
Name: IMP 3 Antibody Clone EP286
Description and applications: IMP-3, known as Insulin-like growth factor 2 (IGF-II) mRNA-binding protein 3, is an oncofetal protein that stabilizes IGF-II mRNA for trafficking and plays an important role in cell growth and migration. This 65- 70 kDa protein is expressed normally in developing tissues during early embryogenesis in a variety of fetal tissues including the liver, lung kidney, thymus, and placenta, but at low or undetectable levels in normal adult tissues. Recent studies have demonstrated IMP-3 expression in various malignant tumors of the lung, gastrointestinal tract, liver, endometrium, and bladder, while undetectable in adjacent benign tissues. IMP-3 may have a critical role in tumor proliferation, invasion and metastasis, and has been suggested to be an independent marker for poor prognosis in patients with clear cell carcinomas.
Composition: Anti-human IMP 3 rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی Inhibin Alpha (R1)
Name: Inhibin Alpha Antibody (Clone R1)
Description and applications: This antibody recognizes the 32kDa alpha subunit of human inhibin. Inhibin alpha is expressed in a range of tissues including prostate, brain, adrenal gland, testis and ovary. The antibody may be of value in the differentiation of adrenocortical tumors, placental and gestational trophoblastic lesions and sex cord stromal tumors.
Composition: anti-human Inhibin alpha mouse monoclonal antibody purified from ascites. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی Insulin (EP125)
Name: Insulin Antibody Clone EP125
Description and applications: Insulin is a hormone that regulates glucose homeostasis. It increases cell permeability to monosaccharides, amino acids and fatty acids, and it accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. It is synthesized in the beta cell of the pancreas. The antibody labels both normal and neoplastic insulinproducing cells. It is useful in identifying insulinoma.
Composition: Anti-human Insulin rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی Involucrin (SY5)
Name: Involucrin Antibody Clone SY5
Description and applications: Involucrin is expressed in a range of stratified squamous epithelia, including the cornea. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures it is expressed by all cells that have left the basal layer. Involucrin expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.
Composition: Anti-human Involucrin mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی KI 67 (SP6)
Name: Rabbit Anti-Human Ki-67 Monoclonal Antibody clone SP6
Description and aplications: This rabbit monoclonal antibody (clone SP6) reacts with a nuclear antigen which is expressed in every proliferating human cell. It is expressed mainly during the cell cycle phases, including late G1, S, G2 and M phase. The cells in the G0 phase (quiescent cells) are not immunostained. This antibody is useful to identify the degree of cell proliferation. Is a marker to define the growth fraction in benign and malignant tissues, such as prostate, breast or lymphoid tissues. Therefore, it can play an important role as a predictor of malignancy.
Composition: anti-Ki67 rabbit monoclonal antibody obtained from supernatant culture and prediluted in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal.
Intended use : Immunohistochemistry (IHC) on paraffin embedded tissues. Not tested on frozen tissues or Western-Blotting
Immunogen: Synthetic peptide derived from the Cterminus of human Ki67 protein
Species reactivity: In vitro diagnostics in humans. Not tested in other species
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی LH (Luteinizing Hormone) (Polyclonal)
Name: LH (Luteinizing Hormone) Antibody (Polyclonal)
Description and applications: This antibody reacts with the human luteinizing hormone (LH). The adenohypophysis produces six main hormones: prolactin (PRL), growth hormone (GH), adrenocorticotropic hormone (ACTH), thyroid stimulating hormone (TSH), follicle-stimulating hormone (FSH) and luteinizing hormone (LH). The
producing cells (approximately 10%) of the two latest hormones are located in the pars tuberalis of the hypophysis. The glycoprotein hormones LH, FSH and TSH are heterodimers with a common alpha subunit and a solely beta subunit that gives a biochemical, immunological and functional specificity as hormone. The LH induces the secretion of sex steroids in the gonads of both sexes; the Leydig cells of the testicle respond synthesizing testosterone and in the ovary, the theca cells respond synthesizing estrogens and inducing ovulation. The luteinizing hormone (LH) is a glycoprotein secreted by both normal and neoplasic hypophysary cells. Therefore, it can be detected in hypophysary adenomas derived from these cells that correspond to chromophobe cells. They represent approximately 6% of hypophysary adenomas.
The LH/FSH secreting adenomas have a slow growth and, at the moment of the diagnosis, are usually large, although they are rarely associated with high serum levels of gonadotropins. The adenomas that only secrete LH are rare. The null cell adenomas and oncocytomas, that do not prove clinically nor biochemically hormone production, show focal expression of LH, FSH, TSH and alpha subunit.Composition: anti-human LH (Luteinizing Hormone) rabbit polyclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and
0.09% sodium azide.Immunogen: Purified luteinizing hormone from the human hypophysis.
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی Cytokeratin 18 (DC-10)
Name: Keratin 18 Antibody Clone DC-10
Description and applications: The nomenclature coined in 1982 by Moll and Franke assigned ranges from 1 to 8 for keratin type II (neutral or basic) and between 9 and 21 for type I (acidic). However and because of the high homology among the different molecules is common for a single monoclonal antibody to react with different types of keratins , for example keratin brand AE1 keratins 10, 14 , 15, 16 and 19. This antibody reacts with the acid intermediate filament protein of 45 kDa keratin, identified as Keratin 18. Keratin 18 is generally co-expressed with the keratin 8 in most simple gland epithelia. In general, the keratin 18 does not react with squamous multilayered epithelium. In neoplastic tissues, this antibody reacts with different benign and malignant epithelial lesions. Most adenocarcinomas and basal cell carcinomas express this keratin, whereas it is absent in squamous cell carcinomas.
Composition: anti-Keratin 18 mouse monoclonal antibody obtained from supernatant culture and prediluted in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal. The amount of the active antibody in the sample is 3,3 μg/mL..
Immunogen: Human breast cancer PMC 42 cells.
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی LIN-28 (EP150)
Name: LIN-28 Antibody Clone EP150
Description and applications: LIN28 is a highly conserved, RNA-binding protein (RBP). It plays an important role as a translational enhancer, leading specific mRNAs to polysomes and therefore increasing the competence of protein synthesis. LIN28 was identified as a negative regulator of miRNA biogenesis and suggested to play a central role in blocking miRNAmediated differentiation in stem cells and certain cancers. LIN28 is expressed by various undifferentiated embryonic cell types. Anti- LIN28 has been used as a sensitive marker for germ cell tumors. The positive staining of LIN28 in yolk sac tumors showed an advantage over OCT4, which is negative in these tumors.
Composition: Anti-human LIN-28 rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی Lysozyme (Polyclonal)
Name: Lysozyme Antibody (Polyclonal)
Description and applications: Lysozyme is one of the anti-microbial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells,
plasma, saliva, and tears. Missense mutations in LYZ have been identified in heritable renal amyloidosis. Lysozyme is synthesized predominantly in reactive histiocytes rather than in resting, unstimulated phagocytes. Anti-lysozyme stains myeloid cells, histiocytes, granulocytes, macrophages, and monocytes. It is an important marker that may demonstrate the myeloid or monocytic nature of acute leukemia. Anti-lysozyme may aid in the identification of histiocytic neoplasias, large lymphocytes, and classifying lymphoproliferative disorders.Composition: anti-human Lysozyme rabbit polyclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی Cytokeratin 5 (SP27)
Name: Cytokeratin 5 Antibody Clone SP27
Description and applications:Twenty human keratins are divided into acidic (pI <5.7) and basic (pI >6.0) subfamilies. Members of the acidic and basic subfamilies are found together in pairs. The composition of keratin pairs varies with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in
cancer research and tumor identification. Point mutations in keratin 5 gene can cause various types of epidermolysis bullosa simplex. Keratin 5 is expressed in most epithelial cells, prostate basal cells and epithelial and biphasic mesotheliomas.Composition: Anti-human Cytokeratin 5 rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
Immunogen: Synthetic peptide from Cterminus of human cytokeratin 5.
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی MAP-2 (AP20)
Name: Mouse anti-human MAP-2 Monoclonal Antibody clone AP20
Description and applications: MAP-2 (microtubule associated protein-2) is one of several high molecular weight proteins that play an important role in brain neuron microtubule assembly. In addition to its association with microtubules, MAP- 2 associates with neurofilaments and actin filaments suggesting that it may guide interaction among microtubules, other cytoskeletal elements, and cytoplasmic organelles. MAP-2 is a stringent marker for neurons. In addition, MAP-2 displays intracellular specificity. In the central nervous system, MAP-2 is confined to neuronal cell bodies and dendrites. There are exceptions, however, where some axons stain positive for small amounts of MAP-2. MAP-2 is uniformly distributed throughout the cell when first expressed in cultured neurons but becomes selectively localized as dendritic development proceeds.
Composition: Anti-human MAP-2 mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide
Intended use: Immunohistochemistry (IHC) on paraffin embedded tissues. Not tested on frozen tissues or Western-Blotting
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی MyoD1 (EP212)
Name: Rabbit anti-human Myo-D1 Monoclonal Antibody clone EP212
Description and applications: MyoD1 is a protein with a key role in regulating muscle differentiation. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. MyoD1 is expressed in developing skeletal muscle tissue but faintly in adult skeletal muscle. In abnormal tissues, it labels tumour cell in rhabdomyosarcoma. MyoD1 is one of the earliest markers of myogenic commitment. Antibody to MyoD1 has been useful to differentiate rhabdomyosarcomas from other tumors. It is a sensitive and specific marker for myogenic differentiation
Composition: anti-human Myo-D1 rabbit monoclonal antibody purified from ascites. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide
Intended use: Immunohistochemistry (IHC) on paraffin embedded tissues. Not tested on frozen tissues or Western-Blotting
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی Mast Cell Tryptase (EP259)
Name: Mast Cell Tryptase Antibody Clone EP259
Description and applications: Human mast cell tryptase, comprises a family of trypsin-like neutral serine proteinases that are predominantly expressed in mast cells. Tryptase has effects on peptides, proteins, cells, and tissues, and many of these actions can ultimately contribute to asthma symptoms. Mast-cell tryptase is found in mast-cell granules and has also been reported to be expressed by peripheral blood basophils at low level. Tryptase has been used as a marker of mast cell activation.
Composition: Anti-human Mast Cell Tryptase rabbit monoclonal antibody purified from serum and prepared in 10mM
PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide. -
آنتی بادیهای ایمونوهیستوشیمیآنتی بادی Myogenin (EP162)
Name: Myogenin-Clone EP162
Description and applications: acid-rich region and a helix-loop-helix (HLH) structure, which can promote muscle development and maintain muscle-specific gene expression by transactivation. Myogenin, one of the myogenic regulatory factors, plays a key role in determining the commitment and differentiation of primitive mesenchymal cells into skeletal muscle. The expression of Myogenin is restricted to cells of skeletal muscle origin, but it is not detected in adult skeletal muscles. It is therefore considered to be an extremely reliable and specific marker for diagnosing rhabdomyosarcomas. Myogenin is positive in virtually 100% of alveolar, sclerosing and botryoides rhabdomyosarcoma and in over 60% of pleomorphic rhabdomyosarcoma and is useful in recognizing adult spindle cell rhabdomyosarcomas. Less than 20% of Wilms tumors, congenital infantile fibrosarcoma, childhood myofibromatosis, synovial sarcoma and desmoid tumors have been reported positive. Ewing sarcoma, rhabdoid tumor, epithelioid sarcoma, leiomyosarcoma, miofibrosarcomas and nodular fasciitis show no reactivity to myogenin.
Composition:anti-human Myogenin rabbit monoclonal antibody purified by protein affinity and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
Immunogen: A synthetic peptide corresponding to residues in human Myogenin was used as an immunogen.