آنتی ­بادی Cytokeratin 18 (DC-10)

Name: Keratin 18 Antibody Clone DC-10

Description and applications: The nomenclature coined in 1982 by Moll and Franke assigned ranges from 1 to 8 for keratin type II (neutral or basic) and between 9 and 21 for type I (acidic). However and because of the high homology among the different molecules is common for a single monoclonal antibody to react with different types of keratins , for example keratin brand AE1 keratins 10, 14 , 15, 16 and 19. This antibody reacts with the acid intermediate filament protein of 45 kDa keratin, identified as Keratin 18. Keratin 18 is generally co-expressed with the keratin 8 in most simple gland epithelia. In general, the keratin 18 does not react with squamous multilayered epithelium. In neoplastic tissues, this antibody reacts with different benign and malignant epithelial lesions. Most adenocarcinomas and basal cell carcinomas express this keratin, whereas it is absent in squamous cell carcinomas.

Composition: anti-Keratin 18 mouse monoclonal antibody obtained from supernatant culture and prediluted in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal. The amount of the active antibody in the sample is 3,3 μg/mL..

Immunogen: Human breast cancer PMC 42 cells.