Name: CD15 Antibody (Clone MMA)
Description and aplications: This antibody reacts against the human antigen CD15 (fucosyltransferase 4) also known as Lewis X (Lex); 3-fucosyl-Nacetyllactosamine [3-FL]; X hapten; specific embryonic antigen-1 to step [SSEA-1]; lacto-N-fucopentaose III
[LNFP III]. This antibody is expressed in normal myelomonocytic cells (90% of human neutrophil granulocytes in peripheral blood and lymphoid and 30% – 60% of circulating monocytes), not being present in normal lymphocytes. CD15 is consistently expressed by the
epithelial cells of renal tubules, gastric glandular epithelium, tonsil and esophageal squamous epithelium, ductal epithelium of salivary glands, pancreas and mammary ducts, thymus Hassall corpuscles, gray and white matter and in endocrine cells of the adrenal cortex and anterior pituitary. In neoplasms, CD15 antigen is expressed by the Reed- Sternberg cells of classic Hodgkin lymphoma (membrane pattern and / or Golgi area), in some leukemias (acute and chronic myeloid leukemia (> 50%), acute myelogenous leukemia (> 95% of chronic phase and> 50% blast phase), and in carcinomas derived from organs whose cells are positive for CD15, including adenocarcinomas of kidney, digestive tract, squamous cell carcinomas. The CD15 may be useful in confirming the diagnosis of Hodgkin’s disease and the differential diagnosis of mesothelioma (usually negative) and lung adenocarcinoma (usually positive).
Composition: anti-human CD15 mouse monoclonal antibody purified from ascites fluid by Protein A chromatography. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.