پروب فیش BCL6 Break Apart FISH Probe

INTRODUCTION: Rearrangements of the BCL6 gene (also known as BCL5, LAZ3, BCL6A, ZNF51 or ZBTB27) have been described in lymphomas and B-cell leukemias. BCL6 is also unregulated in cases of multiple myeloma and several types of solid tumors. More than 30 different genes have been described as translocation pairs. In practice, the presence of the BCL6 translocation may have diagnostic value in follicular lymphomas grade 3B with negative BCL2 translocation, while in diffuse large B-cell lymphomas, along with BCL2 and MYC translocations, it presents a prognostic and therapeutic value.

INTENDED USE: The BCL6 Break Apart FISH probe is designed to detect rearrangements in the BCL6 human gene located in the chromosome band 3q27.3. Aside from detecting breaks that can lead to translocation of parts of the gene, to its inversion or to its fusion with other genes, the whole probe can also be used to identify other aberrations of BCL6, such as deletions or amplifications.

PROBE DESCRIPTION: The 5’ telomeric fragment of the BCL6 Break Apart FISH probe labeled with the fluorochrome CytoOrange covers some sequences adjacent to the 5’ portion (beginning) of the BCL6. The 3’ centromeric fragment of the BCL6 Break apart FISH probe labeled with CytoGreen covers some distal sequences (3’ end) of the gene. Both loci are flanking sequences of the BCL6 gene, in which numerous cut-off points have been observed.