پروب فیش FOXO1 Break Apart FISH Probe

INTRODUCTION: Rearrangements and abnormal expression of the FOXO1 gene (also known as FKH1, FKHR or FOXO1A) have been observed in the alveolar rhabdomyosarcoma, prostate carcinoma and other types of tumors. In the case of alveolar rhabdomyosarcoma, fragments of the 3’ region of the FOXO1 gene translocate with fragments of the 5’ portions of the PAX3 and PAX7 genes, located in the chromosome 2 and 1 respectively.  Those cases showing the PAX3-FOXO1 fusion in metastases present a worse prognosis, while in primary tumors, the development of the disease is independent of the type of translocation. In the prostate cancer, deletions of the FOXO1 gene have been detected in up to 30% of the cases.

INTENDED USE:  The FOXO1 Break Apart FISH probe is designed to detect rearrangements in the FOXO1 human gene located in the band of the chromosome 13q14.11. Aside from detecting breaks that can lead to translocation of parts of the gene, to its inversion or to its fusion with other genes, the probe can also be used to identify other aberrations of the FOXO1 gene such as deletions or amplifications.

PROBE DESCRIPTION: The 5’ telomeric fragment of the FOXO1 Break Apart FISH probe labeled with the fluorochrome CytoOrange covers the center and the 5’ portion (beginning) of the FOXO1 gene and some adjacent genomic sequences. The 3’ centromeric fragment of the FOXO1 Break apart FISH probe labeled with CytoGreen covers the 3’ part (end) of the FOXO1 gene as well as the adjacent and inferior sequences of the gene. Both probes are flanking sequences of the FOXO1 gene, in which variable cut-off points have been observed.