monoclonal

Name: CD163 Antibody (Clone EP324)

Description and aplications: CD163 was recently identified as an acute phase-regulated transmembrane protein whose function is to mediate the endocytosis of haptoglobin-hemoglobin complexes. This receptor is expressed on the surface of monocytes (low expression) and tissue macrophages [also known as histiocytes] (high expression). It is a member of the cysteine-rich scavenger receptor superfamily, encoded by a gene localized on human chromosome 12p13.3. Solubilized in plasma, CD163 functions as an anti-inflammatory signal and has many roles in disease processes that range from autoimmune conditions such as rheumatoid arthritis to atherosclerosis. Previous work has shown that the CD163 gene can be regulated by glucocorticoids, IL-10, and other inflammatory modulators, and is highly expressed in inflamed tissues, consistent with its role in the resolution of inflammation.
Staining for CD163 has been helpful in distinguishing synovial macrophages from synovial intimal fibroblasts in the setting of rheumatoid arthritis,where its specificity for macrophages was found to be superior to that of CD68, which does not discriminate between these cell types. Increased levels of CD163 were also detected in patients with microbial infections and myelomonocytic leukemias by an enzyme-linked immunosorbent assay. Flow cytometry studies have confirmed that CD163 expression is limited to leukemias with monocytic differentiation. Another recent study showed that all 5 cases of synovial-type giant cell tumors of the vertebral column stained for CD163.

Composition: anti-human CD163 mouse monoclonal antibody obtained from supernatant. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Name: CD19 Antibody (Clone SP110)

Description and aplications: This antibody recognizes the CD19, a surface glycoprotein of 95 kD which is present in both malignant and normal B cells which was always been considered as the most reliable surface marker of this cell line, within all range of stages of maturation. In the normal lymphoid tissue, the CD19 staining appears in germinal centers (follicular dendritic cells and B cells) in the mantle zone and as scattered cells within the interfollicular areas with overlapping patterns of immunostaining with CD20 and CD22 cells. However, in contrast to CD20, CD19 is also expressed on pre-B cells. CD19 was detected by flow cytometry in extramedullary plasma cells isolated from human tissue. CD19 positivity appears in the vast majority of B cell neoplasms while the plasma cell neoplasms are always negative. No CD19 stain was detected in Reed- Sternberg cells of Hodgkin’s disease.

Composition: anti-CD19 mouse monoclonal antibody obtained from supernatant culture and prediluted in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal.

Name : Rabbit anti-human CD1a Monoclonal Antibody clone EP80

Description and applications: Cluster of Differentiation 1a (CD1a) is part of a family of major histocompatibility complex (MHC) antigen-like glycoproteins that associate with beta-2- microglobulin. CD1a binds self and non-self lipid and glycolipid antigens, presenting them to T-cell receptors on natural killer T-cells. CD1a antibody labels cortical thymocytes, Langerhans’ cells and dendritic cells. It has been used to identify Langerhans’ cell histiocytosis and precursor T lymphoblastic lymphoma/leukemia.

Composition: Anti-human CD1a rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide

Intended use: Immunohistochemistry (IHC) on paraffin embedded tissues. Not tested on frozen tissues or Western-Blotting

Immunogen: A synthetic peptide corresponding to residues in human CD1a protein.

Name: CD21 Antibody Clone 2G9

Description and applications: The CD21 or CR2 antigen is a 145 kD glycoprotein of molecular weight, which constitutes one of the
membrane molecules involved in the transduction of growth signals into the B cells. Also acts as a receptor for the Epstein Barr virus. This antibody also reacts with a leukocyte membrane epitope (CR2), with molecular weights of 95, 72, 50, 32 and 28 kD. The 28 and 72 kD molecular weight fragments of CR2 constitute the point of attachment of C3d. CD21 antigen is an antigen that is expressed in mature B cells. The antigen is present with a high density in follicular dendritic cells (CFD) of B zones of lymphoid tissue. CD21 moderately stains B cells and strongly follicular dendritic cells in frozen tissue sections, while staining of B cells is reduced or non existent in the paraffin embedded sections. Immunostaining of CFDsin paraffin-embedded sections is as strong as that of frozen tissue sections. CD21 immunohistological analysis of CFD of non- Hodgkin’s lymphomas in paraffin embedded sections reveals the generally dense and highly defined nodular network of CFD in follicular lymphomas and a diffuse, disorganised network in some types of diffuse lymphomas. B-cell precursor lymphomas (lymphoblastic lymphomas), Burkitt’s lymphomas, plasmacytomas, and hairy cell leukemias are
constantly lacking a CFD. In non-Hodgkin’s lymphomas of T cell origin, CFDs are mainly restricted to peripheral T-cell lymphomas of the
angioinmunoblastic type and some cases of pleomorphic lymphomas. T-cell lymphoblastic lymphomas, T-CLL and mycosis fungoides do not have a CFD network. The only category of Hodgkin’s disease that has a CFD-like network is the nodular lymphocyte-predominant
form.

Composition: Anti-human CD21 mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: Recombinant human CD21.

Name: CD27 Antibody Clone EPR8569

Description and applications: CD27 is a glycosylated, type I transmembrane protein with a molecular mass of 50-55 kDa that belongs to the TNF receptor superfamily (TNFRSF7), of which it is its seventh member. This protein is also known as T cell activation antigen, S152 protein and T14 protein. It is encoded by a gene located in the chromosomal region 12p13.31. As a member of the tumour necrosis factor (TNF) receptor family, CD27 plays an important role in cell growth and differentiation as well as in apoptosis regulation. In the former case, CD27 binds to CD70, its ligand, and plays an important role in costimulation of T cell activation and regulation of B cell differentiation and proliferation and immunoglobulin synthesis. The cytoplasmic domains of CD27 have also been shown to interact with TRAF2 and TRAF5 to promote activation of NF-kappa B and SAPK/JNK. Its role inapoptosis is linked to its ability to bind to the proapoptotic SIVA protein; thus, CD27 plays an important role in inducing this cell death process. CD27 is expressed in medullary thymocytes, virtually all mature T cells, memory B cells, NK cells, and a fraction of normal plasma cells but not in virgin B cells or effector T cells. In patients diagnosed with acute lymphoblastic leukaemia (ALL), overexpression of CD27 is associated with a more favourable prognosis and an increased anti-leukaemic immune response by the organism. Immunohistochemical techniques have confirmed the expression of the CD27 antigen in isolated cases of mantle cell lymphoma, Burkitt lymphoma, marginal zone lymphoma, and plasmacytoma. In cases of multiple myeloma, apparently the expression of CD27 has predictive value for patient survival, with an overall three-year survival rate of 92% in positive cases compared to 50% in negative cases. CD27 negativity in cases of hairy-cell leukaemia diagnosed in the spleen has been suggested as a useful tool in the differential diagnosis with other types of B cell leukaemia.

Composition: Anti-human CD27 mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen:Human recombinant protein similar to CD27.

Name: CD278 Antibody Clone SP98

Description and applications: CD278 belongs to the CD28 and CTLA-4 cell-surface receptor family and is highly expressed in activated T cells. It is essential both for efficient interaction between T and B cells and for normal antibody responses to T cell dependent antigens. It enhances all basic T cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B cells.

Composition: Anti-human CD278 rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: Synthetic peptide corresponding to the C-terminus of human CD278.

Name:CD34 Antibody clone QBEnd/10

Description and aplications: The QBEnd10 antibody is a class II monoclonal antibody that recognizes an epitope of CD34 that is resistant to neuraminidase and sensitive to glycoprotease and chymopapain. CD34, a single chain transmembrane glycoprotein, is detected in bone marrow precursors, including normal lymphoid precursors or hematogonias where it may act as lectins ligand of these cells with the bone marrow stroma. CD34 expression disappears from the surface of all haematological series during maturation. Megakaryocytes may show varying positivity for CD34. This antibody shows reactivity with most endothelial
cells, expressed on the luminal surface and interdigitating membrane processes between endothelial cells, but is absent in large venous and arterial vessels. CD34 is also absent in sinusoids of the spleen, liver and placenta. The lymphatic vessels are generally weakly stained. CD34 is also expressed in fibroblast-like dendritic cells localized in liver portal tracts and in Peyer’s patches in wound repair phase. In tumors, CD34 is detected in myeloid blasts of myelodysplastic syndromes and in most cases of acute myeloid leukemias and lymphoblast of the acute lymphoblastic leukemias. Mature B cell lymphomas, B and T cell leukemias are all negative for CD34.
Most vascular tumors and sarcomas including Kaposi hemangiosarcomas are positive for CD34. CD34 is expressed in most cases of dermatofibrosarcoma protuberans (while fibrous histiocytoma is negative), solitary fibrous tumor, lipomas (particularly the spindle cell) and liposarcomas, gastrointestinal stromal (GIST) (strongly positive in 80% of cases coinciding with the expression of CD117) and meningiomas in a variable proportion of cases. Moderate expression was detected in cutaneous leiomyomas and weak staining may be observed in tumors of smooth muscle of the uterus or soft tissue.

Composition: anti-human CD34 mouse monoclonal antibody purified from ascites fluid by Protein A chromatography. Prepared in 10mM PBS, pH 7.4, with
0.2% BSA and 0.09% sodium azide

Immunogen: Detergent solubilized vesicular suspension prepared from a perfusate of human term placenta.

Name: CD38 Antibody Clone 38C03(same as SPC32)

Description and applications: CD38 is a single chain type II integral transmembrane protein, which is highly expressed on thymocytes. It is also present on activated T cells and terminally differentiated B cells (plasma cells). Other reactive cells include NK cells, monocytes, macrophages, and dendritic cells. CD38 may be detected on cells from multiple myeloma, ALL (B and T) and some AML. It is, however, not found on most mature resting peripheral lymphocytes. CD38 functions as a multicatalytic ectoenzyme serving as ADP-ribosyl cyclase, cyclic ADP-ribose hydrolase and possibly NAD+ glycohydrolase or as a cell surface receptor.

Composition: Mouse anti-human CD38 monoclonal antibody is obtained from tissue culture supernatant purified and diluted in 10 mM Phosphate buffered saline (PBS), pH 7.2 containing 1% bovine serum albumin (BSA) and 0.09% sodium azide (NaN3).

Immunogen: Recombinant protein encoding the extracellular domain of human CD38.

Name: CD4 Antibody clone EP204

Description and applications: CD4 is a glycoprotein found on the surface of immune cells such as T helper cells, monocytes, macrophages and dendritic cells. It is a co-receptor that assists the T-cell receptor (TCR) with an antigen-presenting cell and also interacts directly with MHC class Ⅱ molecules on the surface of the antigen-presenting cells using its extracellular domain. In lymphatic tissues, the CD4+ T-cells are seen in large numbers in the parafollicular zone, while scattered cells are found in the germinal centres and mantle zone. CD4 is also demonstrated in hepatic sinusoidal cells, monocytes and monocytes-derived cells but not expressed on B-cells and immature thymocytes. Precursor T-lymphoblastic lymphomas are therefore variable in their expression of CD4. Most mature T-cell lymphomas are CD4 positive with the exception of aggressive NK-cell leukemia and extranodal NK/T-cell lymphoma. CD4 plays an important role in the classification of lymphocytes in inflammatory lesions and malignant lymphomas.

Composition: anti-human CD4 rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: A synthetic peptide corresponding to residues in human CD4 protein.

Name: CD45 (leukocyte common antigen – LCA)Antibody (cocktail of clones 2B11 & PD7/26)

Description and aplications: Anti-CD45 (anti-leukocyte common antigen) is routinely used to aid the differential diagnosis of undifferentiated neoplasms, whenever malignant lymphoma is suspected by the morphological or clinical data. It is a highly specific antibody, therefore a positive result is highly indicative of hematolymphoid origin. Certain types of hematolymphoid neoplasms may lack CD45 (Hodgkin lymphoma, some T-cell lymphomas, and some leukemias) so its absence does not rule out a hematolymphoid tumor. This antibody is expressed almost exclusively by cells of hematopoietic lineage and is present in most benign and malignant lymphocytes as well as plasma cell precursors.

Composition: anti-human CD45 mouse monoclonal antibody purified from ascites fluid by Protein A chromatography. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Name: CD45RO Antibody Clone UCHL-1

Description and applications: The CD45RO antibody recognizes a 180-185kDa protein, identified as an isoform of leukocyte common antigen (CD45RO) (4th Leucocyte Typing Workshop: Code No. N31).The epitope recognized by this antibody is sensitive to neuraminidase digestion. This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells
within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.

Composition: Anti-human CD45RO mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: Cultured human T-cells from an IL-2- dependent T-cell line (CA1).

Name: CD45RB Antibody Clone BRA-11

Description and applications: CD45 has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45 are distributed through-out the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5 and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45, while the intracellular domain is conserved. CD45 functions as a phosphotyrosine phosphatase, a vital component for efficient tyrosine phosphorylation induction by the TCR/CD3 complex. The tyrosine phosphatase activity of CD45 is contained within the conserved intracellular domain. Src and Syk family protein tyrosine kinases are utilized by the TCR/CD3 complex to initiate signaling cascades. Several members of these two families, including Lck, Fyn and ZAP-70, have been implicated as physiological substrates of CD45. CD45RB is expressed in a high proportion of circulating B lymphocytes, suppressor and cytotoxic T lymphocytes, a subpopulation of helper T lymphocytes and in most thymocytes. Expression of CD45RB decreases as the T cells mature from naïve to memory mature T cells, suggesting that its expression is predominantly in immature T lymphocytes. The anti-CD45RB antibody is useful in the study of lymphocyte subpopulations in autoimmune diseases, especially inflammatory bowel disease, and for the immunophenotypic characterization of leukemias and lymphomas.

Composition: Anti-human CD45RB mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.